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- W2063762101 abstract "Abstract An automatic pre-column double derivative procedure has been established for the determination of plasma amino acids by reversed-phase high performance liquid chromatography. O-phthaldialdehyde/3-mercaptopropionic acid and fluorenylmethyl-chloroformate were used as reagents for the derivatization. The optimal conditions for separation and detection and the stability of derivative were investigated. This method has the advantages of automatic pre-column derivatization and analysis; simple and economic mobile phase; shorter analysis time with optimal separation; high precision for retention time and peak area; higher sensitivity and reliable peak identification and simultaneous determination of both primary and secondary amino acids. Plasma amino acid levels in 160 healthy children were determined and the results showed a good agreement with reference values reported previously by using an ion-exchange chromatographic method. Patients suffering from several type of amino acid metabolic abnormality were detected in the screening of selected pediatric patients. This method appears suitable for application to the measurement of plasma amino acids in a clinical laboratory." @default.
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- W2063762101 date "1992-12-01" @default.
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- W2063762101 title "Automatic Pre-Column Derivatization and Reversed-Phase High Performance Liquid Chromatography of Primary and Secondary Amino Acids in Plasma with Photo-Diode Array and Fluorescence Detection" @default.
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- W2063762101 doi "https://doi.org/10.1080/10826079208020887" @default.
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