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- W2063797024 abstract "Protein phosphorylation has been measured after injection of [32P]phosphate into oocytes of Xenopus laevis undergoing progesterone-induced meiotic maturation. As oocytes mature, there is a burst of nonyolk protein phosphorylation several hours after progesterone exposure and shortly before germinal vesicle breakdown (GVBD). This burst is not due to changes in the specific activity of the phosphate or ATP pool. Enucleated oocytes exposed to progesterone also experience the burst, indicating the cytoplasmic location of phosphoprotein formation. When an oocyte receives an injection of cytoplasm containing the maturation-promoting factor (MPF), a burst of protein phosphorylation occurs immediately, and GVBD occurs shortly thereafter, even in the presence of cycloheximide. Under a variety of conditions promoting or blocking maturation, oocytes which undergo GVBD are the only ones to have experienced the phosphorylation burst. The results suggest that the protein phosphorylation burst is a necessary step in the mechanism by which MPF promotes GVBD." @default.
- W2063797024 created "2016-06-24" @default.
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- W2063797024 date "1977-07-01" @default.
- W2063797024 modified "2023-09-27" @default.
- W2063797024 title "Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes" @default.
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- W2063797024 doi "https://doi.org/10.1016/0012-1606(77)90093-8" @default.
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