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- W2063814458 abstract "A previous report from this laboratory showed that two DDT isomers, o,p′-DDT and p,p′-DDD, increased the frequency of spontaneous oscillatory contractions to a similar extent in isolated rat uterine segments. Because regulation of intracellular calcium is fundamental for the development of oscillatory contractions, the present study examined the effects of p,p′-DDD on intracellular free calcium concentration ([Ca2+]i) in individual rat myometrial smooth muscle cells loaded with the fluorescent Ca2+ indicator fura 2. In the presence of extracellular calcium, 50 and 100 μM p,p′-DDD significantly increased peak [Ca2+]i 586 and 921%, respectively, over basal [Ca2+]i. No significant effect was observed with 10 μM p,p′-DDD. In the absence of extracellular calcium, the response to 100 μM p,p′-DDD was significantly attenuated, with cells averaging a 108% increase in peak [Ca2+]i over basal levels, presumably through Ca2+ release from intracellular stores. Nifedipine and cadmium chloride, blockers of voltage-dependent calcium channels, inhibited 100 μM p,p′-DDD-stimulated increases in [Ca2+]i such that peak [Ca2+]i was increased 250% and 259%, respectively. Because of the prominent inhibition observed with the voltage-dependent calcium channel blockers, the effect of p,p′-DDD on membrane depolarization was examined using a cationic fluorescent indicator of membrane potential, [diS-C2(5)]. A concentration of 50 μM p,p′-DDD depolarized the cells by 35% of maximum during treatment with p,p′-DDD. The data demonstrate that p,p′-DDD increased [Ca2+]i in rat myometrial smooth muscle cells in a concentration-related manner, and that this increase was largely dependent on influx of extracellular calcium through dihydropyridine-sensitive, voltage-dependent calcium channels. The data further show that p,p′-DDD depolarized the plasma membrane, providing a possible mechanism for activation of voltage-dependent calcium channels. Additionally, another calcium source, perhaps an intracellular pool, contributes significantly less to the rise of [Ca2+]i. Whether p,p′-DDD initiates the calcium response by direct actions on the plasma membrane or by other means remains to be determined." @default.
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- W2063814458 date "1995-11-01" @default.
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- W2063814458 title "The Chlorinated Insecticide 1,1-Dichloro-2,2-bis(4-chlorophenyl)ethane (p,p′-DDD) Increases Intracellular Calcium in Rat Myometrial Smooth Muscle Cells" @default.
- W2063814458 doi "https://doi.org/10.1006/taap.1995.1217" @default.
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