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- W2063844869 abstract "To analyse the effects of 1-day disposable contact lens (CL) material on mononuclear cell activation antigens and adhesion molecules, in an in vitro model set up in order to simulate the first 2 h of wear.Etafilcon A and Hilafilcon A materials were analysed. Mononuclear cells, collected from peripheral blood samples of normal human donors, were placed in contact with the materials, both new and coated with artificial tear solution. As a control, Thermanox coverslips and a group with no material were included. Immunofluorescence reactions were carried out on cells, to study the distribution of activation (HLA-DR, CD25, CD69) and adhesion (CD11a, CD18, CD54) molecules; the flow cytometry technique was used to analyse cells.Statistically significant results were demonstrated for the CD69 and all the 3 adhesion molecules. Artificial tear coating enhanced the mononuclear cell response.The daily mononuclear cell activation can lead, with time, to production of inflammatory mediators that may be responsible for discomfort or, at worst, intolerance and definitely to CL wear remittance." @default.
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- W2063844869 date "2003-01-01" @default.
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- W2063844869 title "Mononuclear Cell Antigen Expression after Contact with One-Day Disposable Contact Lens Materials" @default.
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- W2063844869 doi "https://doi.org/10.1159/000068970" @default.
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