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• W2063884462 abstract "Oropharyngeal candidiasis is emerging as a serious health problem in the elderly as well as other chronically immunosuppressed patient populations. Several approaches have been used to study the interactions between Candida and the oral mucosa in vitro. Recently, three-dimensional organotypic systems of the oral mucosa have been developed, which provide an organizational complexity that is between the culture of single cell types and organ cultures in vivo. In this report we describe the development of a novel three-dimensional system of the human oral mucosa based on an immortalized oral keratinocyte cell line. Unlike the commercially available cell line systems, this system also contains a connective tissue cell component, which ensures the quality and resemblance of the tissue model to the human oral masticatory mucosa and submucosa. Using a panel of Candida albicans strains with variable virulence in vivo, we showed that the extent of tissue damage, fungal invasion and host inflammatory response in this system was proportional to the well-documented in vivo virulence potential of these strains. Therefore, this investigation has added another useful tool in the study of host pathogen interactions in oral candidiasis." @default.
• W2063884462 created "2016-06-24" @default.
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• W2063884462 creator A5033283146 @default.
• W2063884462 date "2006-06-01" @default.
• W2063884462 modified "2023-10-01" @default.
• W2063884462 title "Development of a novel three-dimensional in vitro model of oral Candida infection" @default.
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• W2063884462 doi "https://doi.org/10.1016/j.micpath.2006.02.004" @default.
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