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- W2063896130 abstract "BACKGROUND : Umbilical cord blood processing with standard centrifugation techniques is performed in open systems and results in varying cell and volume recoveries. STUDY DESIGN AND METHODS : Forty umbilical cord blood donations were randomly assigned to processing either with a microprocessor‐controlled cell separator equipped with closed disposables or with a manual separation procedure in blood bags. The collection efficiency of nucleated cells, MNCs, RBCs, and CD34+ cells and the processing time were analyzed. RESULTS : Using the cell processor, mean collection efficiencies were 78.6 ± 24.9 percent for nucleated cells, 77.4 ± 27.8 percent for MNCs, 55.5 ± 14.6 percent for RBCs, and 83.6 ± 32.5 percent for CD34+ cells, while they were 73.1 ± 13.2 percent for nucleated cells, 78.1 ± 14.9 percent for MNCs, 26.0 ± 12.2 percent for RBCs, and 77.0 ± 17.6 percent for CD34+ cells when using the standard centrifugation technique. The processing time was about 20 minutes for automated processing and 60 to 80 minutes for the standard centrifugation technique. CONCLUSION : Using the new cell processor, the collection efficiencies for nucleated cells, MNCs, and CD34+ cells are similar to those obtained by established centrifugation techniques while the RBC reduction is less effective. The main advantages of the new systems are the closed system, the more standardized processing procedure, and a significantly shorter processing time." @default.
- W2063896130 created "2016-06-24" @default.
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- W2063896130 date "2003-05-21" @default.
- W2063896130 modified "2023-10-15" @default.
- W2063896130 title "Cord blood processing with an automated and functionally closed system" @default.
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- W2063896130 doi "https://doi.org/10.1046/j.1537-2995.2003.00398.x" @default.
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