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- W2063905235 abstract "Recent epidemics of human listeriosis have shown the importance of Listeria monocytogenes as a food-borne risk. We have developed an automated identification assay for L. monocytogenes using the specificity of the 16S rDNA probe described by Wang et al. (1991). Bacterial cultures were lysed quickly by a chemical step releasing the target nucleic acids. Extracts were loaded into the VIDAS automate (bioMérieux) which performed a non-radio-active hybridisation reaction for 2 h. This assay recognised specifically 68 out of 69 L. monocytogenes isolates from clinical and food origins, including serovars 4b and 1/2, without cross-hybridising to other Listeria species (103 strains) or other bacterial species (15 strains). The sensitivity of the assay was 10(7) bacteria. This automated technology is faster than conventional biochemical identification." @default.
- W2063905235 created "2016-06-24" @default.
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- W2063905235 date "1996-01-01" @default.
- W2063905235 modified "2023-09-27" @default.
- W2063905235 title "Automated RNA probe assay for the identification of Listeria monocytogenes" @default.
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- W2063905235 doi "https://doi.org/10.1016/0168-1605(95)00003-8" @default.
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