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- W2063917154 abstract "Sections of resin embedded, aldehyde fixed T4 bacteriophages, adsorbed on bacterial envelopes, are observed unstained with different imaging modes and their surface reliefs analysed by heavy metal shadowing. It is found that details seen in bright-field and dark-field imaging reflect strongly the features of the relief. Most biological materials situated in the interior of the slice are not visible in bright-field; illustrating the few exceptions, the compact DNA of a bacterial virus that spans the entire section is visible by virtue of its density difference. By using resins with a chemically incorporated heavy metal the contrast is increased, as is the visibility of nearest neighbour distortions of the resin around the biological structures. The distortions studied with such a resin easily explain the blur observed with dark-field imaging in STEM of unstained sections with normal resin. It is confirmed that this blurring effect is eliminated either by ratio-contrast imaging that is less sensitive to thickness variations than the conventional modes or, obviously, by the abundant heavy metal stain commonly used. We then compared the experimental results with contrast calculations made previously and obtained excellent agreement between theory and experiment." @default.
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- W2063917154 date "1986-01-01" @default.
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- W2063917154 title "The influence of the surface relief of thin sections of embedded, unstained biological material on image quality" @default.
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- W2063917154 doi "https://doi.org/10.1016/0739-6260(86)90026-2" @default.
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