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- W2064039733 abstract "The fluorescent probe 1,1′-dihexyl-2,2′-oxacarbocyanine was used to measure the membrane potential in fermenting Streptococcuslactis 7962 cells. To calibrate the fluorescence changes and the membrane potential, the decrease in fluorescence of this dye was measured in valinomycin-treated cells and related to the membrane potential calculated with the Nernst equation at various K+ ratios in/out. The membrane potential observed when cells ferment glucose or arginine was determined using such calibration curves. The proton-motive force during fermentation was calculated from the membrane potential and the pH gradient (measured by the distribution of 5,5-dimethyl-2,4-oxazolidinedione). The values obtained for the proton-motive force were found to correlate directly with the thiomethyl-β-galactoside accumulation in glucose- or arginine-energized cells. These data are in accordance with the chemiosmotic view of coupling of metabolic energy to the active transport of nutrients via electrochemical proton gradients." @default.
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- W2064039733 date "1974-08-01" @default.
- W2064039733 modified "2023-09-23" @default.
- W2064039733 title "Protonmotive force in fermenting Streptococcuslactis 7962 in relation to sugar accumulation" @default.
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- W2064039733 doi "https://doi.org/10.1016/s0006-291x(74)80061-6" @default.
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