Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064044967> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2064044967 endingPage "49" @default.
- W2064044967 startingPage "45" @default.
- W2064044967 abstract "The secretion of Bacillus subtilis (Bs) levanase (Lev) was studied in the yeast Saccharomyces cerevisiae. A set of different yeast expression plasmids, based on the constitutive PGK promoter and harbouring the Bs Lev-encoding gene (sacC), was constructed. In these plasmids, the original Bs signal sequence was either intact, partially deleted or entirely missing. With all constructs, Lev was produced from yeast transformants. However, only when the intact bacterial signal peptide was present was the synthesized enzyme secreted; around 20% was found in the periplasm and 30% in the culture medium. The secreted protein found in the periplasmic space was mainly core-glycosylated and unglycosylated, and had a size of 80-90 and 74 kDa, respectively. In contrast, Lev found in the culture medium was mainly hyper-glycosylated and had a size of 180-200 kDa. Yeast transformants harbouring sacC, but lacking parts of the bacterial signal sequence, only produced cytoplasmic protein which was not glycosylated and had a size of about 74 kDa. The deletion of the entire signal peptide and a further 22 amino acids at the N terminus of mature Lev resulted in a 71-kDa cytoplasmic protein which was not active." @default.
- W2064044967 created "2016-06-24" @default.
- W2064044967 creator A5014950513 @default.
- W2064044967 creator A5024296433 @default.
- W2064044967 creator A5040763775 @default.
- W2064044967 date "1995-01-01" @default.
- W2064044967 modified "2023-10-13" @default.
- W2064044967 title "Efficient secretion of bacillus subtilis levanase by saccharomyces cerevisiae" @default.
- W2064044967 cites W1523637574 @default.
- W2064044967 cites W1593184463 @default.
- W2064044967 cites W1966642263 @default.
- W2064044967 cites W1988394498 @default.
- W2064044967 cites W2001019297 @default.
- W2064044967 cites W2007833212 @default.
- W2064044967 cites W2019410656 @default.
- W2064044967 cites W2020342479 @default.
- W2064044967 cites W2020659453 @default.
- W2064044967 cites W2057032836 @default.
- W2064044967 cites W2057188804 @default.
- W2064044967 cites W2082989069 @default.
- W2064044967 cites W2083899083 @default.
- W2064044967 cites W2100837269 @default.
- W2064044967 cites W2126135568 @default.
- W2064044967 cites W2134258961 @default.
- W2064044967 cites W2062853073 @default.
- W2064044967 doi "https://doi.org/10.1016/0378-1119(95)00284-d" @default.
- W2064044967 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7642135" @default.
- W2064044967 hasPublicationYear "1995" @default.
- W2064044967 type Work @default.
- W2064044967 sameAs 2064044967 @default.
- W2064044967 citedByCount "9" @default.
- W2064044967 countsByYear W20640449672012 @default.
- W2064044967 countsByYear W20640449672023 @default.
- W2064044967 crossrefType "journal-article" @default.
- W2064044967 hasAuthorship W2064044967A5014950513 @default.
- W2064044967 hasAuthorship W2064044967A5024296433 @default.
- W2064044967 hasAuthorship W2064044967A5040763775 @default.
- W2064044967 hasConcept C104317684 @default.
- W2064044967 hasConcept C10858879 @default.
- W2064044967 hasConcept C153911025 @default.
- W2064044967 hasConcept C156399914 @default.
- W2064044967 hasConcept C167625842 @default.
- W2064044967 hasConcept C201663137 @default.
- W2064044967 hasConcept C22744801 @default.
- W2064044967 hasConcept C2777272437 @default.
- W2064044967 hasConcept C2777576037 @default.
- W2064044967 hasConcept C2779190341 @default.
- W2064044967 hasConcept C2779222958 @default.
- W2064044967 hasConcept C49039625 @default.
- W2064044967 hasConcept C523546767 @default.
- W2064044967 hasConcept C54355233 @default.
- W2064044967 hasConcept C547475151 @default.
- W2064044967 hasConcept C55493867 @default.
- W2064044967 hasConcept C86803240 @default.
- W2064044967 hasConceptScore W2064044967C104317684 @default.
- W2064044967 hasConceptScore W2064044967C10858879 @default.
- W2064044967 hasConceptScore W2064044967C153911025 @default.
- W2064044967 hasConceptScore W2064044967C156399914 @default.
- W2064044967 hasConceptScore W2064044967C167625842 @default.
- W2064044967 hasConceptScore W2064044967C201663137 @default.
- W2064044967 hasConceptScore W2064044967C22744801 @default.
- W2064044967 hasConceptScore W2064044967C2777272437 @default.
- W2064044967 hasConceptScore W2064044967C2777576037 @default.
- W2064044967 hasConceptScore W2064044967C2779190341 @default.
- W2064044967 hasConceptScore W2064044967C2779222958 @default.
- W2064044967 hasConceptScore W2064044967C49039625 @default.
- W2064044967 hasConceptScore W2064044967C523546767 @default.
- W2064044967 hasConceptScore W2064044967C54355233 @default.
- W2064044967 hasConceptScore W2064044967C547475151 @default.
- W2064044967 hasConceptScore W2064044967C55493867 @default.
- W2064044967 hasConceptScore W2064044967C86803240 @default.
- W2064044967 hasIssue "1" @default.
- W2064044967 hasLocation W20640449671 @default.
- W2064044967 hasLocation W20640449672 @default.
- W2064044967 hasOpenAccess W2064044967 @default.
- W2064044967 hasPrimaryLocation W20640449671 @default.
- W2064044967 hasRelatedWork W1675317088 @default.
- W2064044967 hasRelatedWork W1728286703 @default.
- W2064044967 hasRelatedWork W2002594144 @default.
- W2064044967 hasRelatedWork W2082719373 @default.
- W2064044967 hasRelatedWork W2097662509 @default.
- W2064044967 hasRelatedWork W2121394713 @default.
- W2064044967 hasRelatedWork W2142210757 @default.
- W2064044967 hasRelatedWork W2913872601 @default.
- W2064044967 hasRelatedWork W4283802666 @default.
- W2064044967 hasRelatedWork W65679617 @default.
- W2064044967 hasVolume "161" @default.
- W2064044967 isParatext "false" @default.
- W2064044967 isRetracted "false" @default.
- W2064044967 magId "2064044967" @default.
- W2064044967 workType "article" @default.