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• W2064123559 abstract "Two strains of the Leishmania braziliensis complex have been adapted to grow extracellularly at elevated temperature as amastigote-like forms in a cell-free medium. These parasites can be serially cultivated and maintained at 32 degrees C for L. panamensis (WR442; L. braziliensis panamensis) and at 28 degrees C for L. braziliensis (M5052; L. braziliensis braziliensis). Several observations are presented that the forms adapted at elevated temperature are amastigote-like. Morphologically, the amastigote-like organisms appear rounded to ovoid and are immotile and smaller than promastigotes; the flagellum of the amastigote-like forms does not extend beyond the flagellar pocket. In comparison, the promastigotes are very elongated, with a nucleus at mid-cell length and a very long flagellum. By electron microscopy, the short flagellum of the amastigote-like form is within a distended flagellar pocket; the 9 + 2 axonemal configuration is present but the paraxial rod is not observed. By contrast, the flagellum of the promastigote has a paraxial rod which extends from the axosome level. In addition, these amastigote-like forms of Leishmania are able to infect, to survive and to divide within the macrophage cell line J774." @default.
• W2064123559 created "2016-06-24" @default.
• W2064123559 creator A5020985367 @default.
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• W2064123559 date "1989-09-01" @default.
• W2064123559 modified "2023-10-13" @default.
• W2064123559 title "I. Extracellular Cultivation and Morphological Characterization of Amastigote-Like Forms of<i>Leishmania panamensis</i>and<i>L. braziliensis</i>" @default.
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• W2064123559 doi "https://doi.org/10.1111/j.1550-7408.1989.tb01086.x" @default.
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