FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064133567> ?p ?o ?g. }

• W2064133567 endingPage "4277" @default.
• W2064133567 startingPage "4271" @default.
• W2064133567 abstract "Three-dimensional (3D) cell culture is regarded as a more physiologically relevant method of growing cells in the laboratory compared to traditional monolayer cultures. Recently, the application of polystyrene-based scaffolds produced using polyHIPE technology (porous polymers derived from high internal phase emulsions) for routine 3D cell culture applications has generated very promising results in terms of improved replication of native cellular function in the laboratory. These materials, which are now available as commercial scaffolds, are superior to many other 3D cell substrates due to their high porosity, controllable morphology, and suitable mechanical strength. However, until now there have been no reports describing the surface-modification of these materials for enhanced cell adhesion and function. This study, therefore, describes the surface functionalization of these materials with galactose, a carbohydrate known to specifically bind to hepatocytes via the asialoglycoprotein receptor (ASGPR), to further improve hepatocyte adhesion and function when growing on the scaffold. We first modify a typical polystyrene-based polyHIPE to produce a cell culture scaffold carrying pendent activated-ester functionality. This was achieved via the incorporation of pentafluorophenyl acrylate (PFPA) into the initial styrene (STY) emulsion, which upon polymerization formed a polyHIPE with a porosity of 92% and an average void diameter of 33 μm. Histological analysis showed that this polyHIPE was a suitable 3D scaffold for hepatocyte cell culture. Galactose-functionalized scaffolds were then prepared by attaching 2'-aminoethyl-β-D-galactopyranoside to this PFPA functionalized polyHIPE via displacement of the labile pentafluorophenyl group, to yield scaffolds with approximately ca. 7-9% surface carbohydrate. Experiments with primary rat hepatocytes showed that cellular albumin synthesis was greatly enhanced during the initial adhesion/settlement period of cells on the galactose-functionalized material, suggesting that the surface carbohydrates are accessible and selective to cells entering the scaffold. This porous polymer scaffold could, therefore, have important application as a 3D scaffold that offers enhanced hepatocyte adhesion and functionality." @default.
• W2064133567 created "2016-06-24" @default.
• W2064133567 creator A5014007107 @default.
• W2064133567 creator A5033884159 @default.
• W2064133567 creator A5049353521 @default.
• W2064133567 creator A5052230032 @default.
• W2064133567 creator A5055161383 @default.
• W2064133567 creator A5077784693 @default.
• W2064133567 date "2013-11-14" @default.
• W2064133567 modified "2023-10-17" @default.
• W2064133567 title "Galactose-Functionalized PolyHIPE Scaffolds for Use in Routine Three Dimensional Culture of Mammalian Hepatocytes" @default.
• W2064133567 cites W1967059698 @default.
• W2064133567 cites W1973223324 @default.
• W2064133567 cites W1975833234 @default.
• W2064133567 cites W1980573164 @default.
• W2064133567 cites W1982769493 @default.
• W2064133567 cites W1984242958 @default.
• W2064133567 cites W1986492406 @default.
• W2064133567 cites W1998663567 @default.
• W2064133567 cites W2009700793 @default.
• W2064133567 cites W2010251891 @default.
• W2064133567 cites W2019168090 @default.
• W2064133567 cites W2022943495 @default.
• W2064133567 cites W2023102326 @default.
• W2064133567 cites W2023416228 @default.
• W2064133567 cites W2026725642 @default.
• W2064133567 cites W2028919654 @default.
• W2064133567 cites W2030224450 @default.
• W2064133567 cites W2031018047 @default.
• W2064133567 cites W2031650452 @default.
• W2064133567 cites W2033474014 @default.
• W2064133567 cites W2041458399 @default.
• W2064133567 cites W2044241207 @default.
• W2064133567 cites W2046061689 @default.
• W2064133567 cites W2074865082 @default.
• W2064133567 cites W2075124376 @default.
• W2064133567 cites W2080252554 @default.
• W2064133567 cites W2081672582 @default.
• W2064133567 cites W2084988572 @default.
• W2064133567 cites W2089889928 @default.
• W2064133567 cites W2092636292 @default.
• W2064133567 cites W2100426249 @default.
• W2064133567 cites W2104614346 @default.
• W2064133567 cites W2116042310 @default.
• W2064133567 cites W2116830628 @default.
• W2064133567 cites W2125903057 @default.
• W2064133567 cites W2145816248 @default.
• W2064133567 cites W2148288287 @default.
• W2064133567 cites W2171534546 @default.
• W2064133567 cites W2327989903 @default.
• W2064133567 doi "https://doi.org/10.1021/bm401145x" @default.
• W2064133567 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3859181" @default.
• W2064133567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24180291" @default.
• W2064133567 hasPublicationYear "2013" @default.
• W2064133567 type Work @default.
• W2064133567 sameAs 2064133567 @default.
• W2064133567 citedByCount "51" @default.
• W2064133567 countsByYear W20641335672014 @default.
• W2064133567 countsByYear W20641335672015 @default.
• W2064133567 countsByYear W20641335672016 @default.
• W2064133567 countsByYear W20641335672017 @default.
• W2064133567 countsByYear W20641335672018 @default.
• W2064133567 countsByYear W20641335672019 @default.
• W2064133567 countsByYear W20641335672020 @default.
• W2064133567 countsByYear W20641335672021 @default.
• W2064133567 countsByYear W20641335672022 @default.
• W2064133567 crossrefType "journal-article" @default.
• W2064133567 hasAuthorship W2064133567A5014007107 @default.
• W2064133567 hasAuthorship W2064133567A5033884159 @default.
• W2064133567 hasAuthorship W2064133567A5049353521 @default.
• W2064133567 hasAuthorship W2064133567A5052230032 @default.
• W2064133567 hasAuthorship W2064133567A5055161383 @default.
• W2064133567 hasAuthorship W2064133567A5077784693 @default.
• W2064133567 hasBestOaLocation W20641335672 @default.
• W2064133567 hasConcept C115537861 @default.
• W2064133567 hasConcept C11569505 @default.
• W2064133567 hasConcept C12554922 @default.
• W2064133567 hasConcept C127413603 @default.
• W2064133567 hasConcept C136229726 @default.
• W2064133567 hasConcept C147789679 @default.
• W2064133567 hasConcept C1491633281 @default.
• W2064133567 hasConcept C163688615 @default.
• W2064133567 hasConcept C178790620 @default.
• W2064133567 hasConcept C185592680 @default.
• W2064133567 hasConcept C188027245 @default.
• W2064133567 hasConcept C192562407 @default.
• W2064133567 hasConcept C202751555 @default.
• W2064133567 hasConcept C2776200302 @default.
• W2064133567 hasConcept C2777922577 @default.
• W2064133567 hasConcept C42360764 @default.
• W2064133567 hasConcept C49892992 @default.
• W2064133567 hasConcept C521977710 @default.
• W2064133567 hasConcept C55493867 @default.
• W2064133567 hasConcept C71924100 @default.
• W2064133567 hasConcept C84416704 @default.
• W2064133567 hasConcept C85789140 @default.
• W2064133567 hasConcept C86803240 @default.
• W2064133567 hasConcept C89429830 @default.

• next