FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064240041> ?p ?o ?g. }

• W2064240041 endingPage "518" @default.
• W2064240041 startingPage "507" @default.
• W2064240041 abstract "The trfA and trb operons of broad host range plasmid RK2 are required for replication and conjugative transfer, respectively. Transcription of the trb operon can be initiated at one of two promoters: trbAp or trbBp. trbBp provides a burst of trb transcription on first entry into the cell. trbAp appears to be responsible for steady-state transcription of the trb operon as well as trbA, encoding a repressor which helps to shut down trbBp. The promoters trfAp and trbAp are arranged as face-to-face divergent promoters. trfAp is very strong and shuts off trbAp activity until trfAp is inhibited by KorA, one of the plasmid-encoded global regulators. Although trfAp is also repressed by KorB, a second global regulator encoded along with KorA in the central control operon, trbAp activation only occurs when KorA is present. KorB did not activate trbAp and indeed had a significant inhibitory effect on KorA activation. In vitro trfAp binds RNA polymerease (RNAP) approximately ten times more strongly than trbAp. Comparison of single and multiple rounds of in vitro run-off transcription suggested that the inhibitory effect of trfAp is due to elongating transcription complexes. In vitro studies with purified KorA and KorB on RNAP binding, isomerization and in vitro transcription suggested that both proteins can displace RNAP from trfAp, but that once open complexes have formed at either promoter they have a good chance of generating a transcript even if they encounter an opposing RNAP. In vivo KorB repressed trbAp even when trbAp was derepressed by a trfAp-1 mutation, removing the need for KorA. This suggested that KorB not only fails to derepress but actually represses trbAp despite the KorB operator being located 90bp downstream of the transcription start point (tsp). By contrast KorA still activated trbAp when the two promoters were moved further apart or were brought so close that RNAP binding to the two promoters was mutually exclusive. Thus, KorA plays the dominant role in achieving the balance of expression of genes for alternate modes of plasmid propagation but its action is modulated by KorB." @default.
• W2064240041 created "2016-06-24" @default.
• W2064240041 creator A5023961871 @default.
• W2064240041 creator A5026991486 @default.
• W2064240041 date "1997-01-01" @default.
• W2064240041 modified "2023-09-26" @default.
• W2064240041 title "Dissection of the switch between genes for replication and transfer of promiscuous plasmid RK2: basis of the dominance of trfAp over trbAp and specificity for KorA in controlling the switch" @default.
• W2064240041 cites W1506043065 @default.
• W2064240041 cites W1537554912 @default.
• W2064240041 cites W1541473088 @default.
• W2064240041 cites W1553714112 @default.
• W2064240041 cites W1650720892 @default.
• W2064240041 cites W1730089026 @default.
• W2064240041 cites W1866208838 @default.
• W2064240041 cites W1895988065 @default.
• W2064240041 cites W1945265201 @default.
• W2064240041 cites W1967082011 @default.
• W2064240041 cites W1970648090 @default.
• W2064240041 cites W1974985494 @default.
• W2064240041 cites W1987889125 @default.
• W2064240041 cites W1995122455 @default.
• W2064240041 cites W2006410528 @default.
• W2064240041 cites W2018181625 @default.
• W2064240041 cites W2018675618 @default.
• W2064240041 cites W2019410656 @default.
• W2064240041 cites W2039008143 @default.
• W2064240041 cites W2044155415 @default.
• W2064240041 cites W2050108888 @default.
• W2064240041 cites W2050325588 @default.
• W2064240041 cites W2050796343 @default.
• W2064240041 cites W2068419274 @default.
• W2064240041 cites W2075422091 @default.
• W2064240041 cites W2086536186 @default.
• W2064240041 cites W2138104394 @default.
• W2064240041 cites W2138270253 @default.
• W2064240041 cites W2142547804 @default.
• W2064240041 cites W2148312059 @default.
• W2064240041 cites W296555646 @default.
• W2064240041 doi "https://doi.org/10.1006/jmbi.1996.0747" @default.
• W2064240041 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9048945" @default.
• W2064240041 hasPublicationYear "1997" @default.
• W2064240041 type Work @default.
• W2064240041 sameAs 2064240041 @default.
• W2064240041 citedByCount "26" @default.
• W2064240041 countsByYear W20642400412013 @default.
• W2064240041 countsByYear W20642400412014 @default.
• W2064240041 countsByYear W20642400412016 @default.
• W2064240041 countsByYear W20642400412020 @default.
• W2064240041 countsByYear W20642400412021 @default.
• W2064240041 countsByYear W20642400412022 @default.
• W2064240041 crossrefType "journal-article" @default.
• W2064240041 hasAuthorship W2064240041A5023961871 @default.
• W2064240041 hasAuthorship W2064240041A5026991486 @default.
• W2064240041 hasConcept C101762097 @default.
• W2064240041 hasConcept C104317684 @default.
• W2064240041 hasConcept C138885662 @default.
• W2064240041 hasConcept C143065580 @default.
• W2064240041 hasConcept C150194340 @default.
• W2064240041 hasConcept C153911025 @default.
• W2064240041 hasConcept C158448853 @default.
• W2064240041 hasConcept C179926584 @default.
• W2064240041 hasConcept C203075996 @default.
• W2064240041 hasConcept C22744801 @default.
• W2064240041 hasConcept C41895202 @default.
• W2064240041 hasConcept C54355233 @default.
• W2064240041 hasConcept C86339819 @default.
• W2064240041 hasConcept C86803240 @default.
• W2064240041 hasConcept C95444343 @default.
• W2064240041 hasConceptScore W2064240041C101762097 @default.
• W2064240041 hasConceptScore W2064240041C104317684 @default.
• W2064240041 hasConceptScore W2064240041C138885662 @default.
• W2064240041 hasConceptScore W2064240041C143065580 @default.
• W2064240041 hasConceptScore W2064240041C150194340 @default.
• W2064240041 hasConceptScore W2064240041C153911025 @default.
• W2064240041 hasConceptScore W2064240041C158448853 @default.
• W2064240041 hasConceptScore W2064240041C179926584 @default.
• W2064240041 hasConceptScore W2064240041C203075996 @default.
• W2064240041 hasConceptScore W2064240041C22744801 @default.
• W2064240041 hasConceptScore W2064240041C41895202 @default.
• W2064240041 hasConceptScore W2064240041C54355233 @default.
• W2064240041 hasConceptScore W2064240041C86339819 @default.
• W2064240041 hasConceptScore W2064240041C86803240 @default.
• W2064240041 hasConceptScore W2064240041C95444343 @default.
• W2064240041 hasIssue "5" @default.
• W2064240041 hasLocation W20642400411 @default.
• W2064240041 hasLocation W20642400412 @default.
• W2064240041 hasOpenAccess W2064240041 @default.
• W2064240041 hasPrimaryLocation W20642400411 @default.
• W2064240041 hasRelatedWork W1895988065 @default.
• W2064240041 hasRelatedWork W1973673615 @default.
• W2064240041 hasRelatedWork W2011715107 @default.
• W2064240041 hasRelatedWork W201602656 @default.
• W2064240041 hasRelatedWork W2022585921 @default.
• W2064240041 hasRelatedWork W2049866186 @default.
• W2064240041 hasRelatedWork W2110270806 @default.
• W2064240041 hasRelatedWork W2134268016 @default.
• W2064240041 hasRelatedWork W2164416211 @default.
• W2064240041 hasRelatedWork W2775143774 @default.

• next