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• W2064298949 endingPage "534" @default.
• W2064298949 startingPage "522" @default.
• W2064298949 abstract "Previous studies demonstrated that DNA and DNA polymerase form a complex at low but not high ionic strength. The data presented in this study show that the complex formed at low ionic strength is converted, by ultraviolet radiation, to a form that is stable to high ionic strength, to heat and to 0.1 M NaOH. The ultraviolet-induced stable complex of the alternating copolymer poly(dA-dT) and DNA polymerase is heterogeneous in CsCl gradients and contains approximately one molecule of DNA polymerase per molecule of poly(dA-dT). The ultraviolet-induced stable complex of Escherichia coli DNA and DNA polymerase is more homogeneous in CsCl gradients and contains 1.6 molecules of DNA polymerase per molecule of double-stranded E. coli DNA. Studies with homopolymers indicate the ultraviolet-induced joining is not base specific. The formation of the complex is proportional to ultraviolet dose and to concentration of DNA polymerase at a given concentration of DNA or poly(dA-dT). Under optimum conditions 65 % of the E. coli DNA or 28 % of the poly(dA-dT) could be obtained as stable complexes with DNA polymerase." @default.
• W2064298949 created "2016-06-24" @default.
• W2064298949 creator A5060435964 @default.
• W2064298949 date "1972-11-01" @default.
• W2064298949 modified "2023-10-18" @default.
• W2064298949 title "Ultraviolet light-induced stable complexes of DNA and DNA polymerase" @default.
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• W2064298949 doi "https://doi.org/10.1016/0005-2787(72)90153-0" @default.
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