FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064308921> ?p ?o ?g. }

• W2064308921 endingPage "328" @default.
• W2064308921 startingPage "321" @default.
• W2064308921 abstract "Protein engineering is becoming increasingly important for pharmaceutical applications where controlling the specificity and affinity of engineered proteins is required to create targeted protein therapeutics. Affinity increases of several thousand-fold are now routine for a variety of protein engineering approaches, and the structural and energetic bases of affinity maturation have been investigated in a number of such cases. Previously, a 3-million-fold affinity maturation process was achieved in a protein–protein interaction composed of a variant T-cell receptor fragment and a bacterial superantigen. Here, we present the molecular basis of this affinity increase. Using X-ray crystallography, shotgun reversion/replacement scanning mutagenesis, and computational analysis, we describe, in molecular detail, a process by which extrainterfacial regions of a protein complex can be rationally manipulated to significantly improve protein engineering outcomes." @default.
• W2064308921 created "2016-06-24" @default.
• W2064308921 creator A5015133421 @default.
• W2064308921 creator A5037693404 @default.
• W2064308921 creator A5046287489 @default.
• W2064308921 creator A5057683371 @default.
• W2064308921 creator A5060558357 @default.
• W2064308921 creator A5066594495 @default.
• W2064308921 creator A5079686174 @default.
• W2064308921 creator A5084369008 @default.
• W2064308921 creator A5088135588 @default.
• W2064308921 date "2011-08-01" @default.
• W2064308921 modified "2023-09-30" @default.
• W2064308921 title "Molecular Basis of a Million-Fold Affinity Maturation Process in a Protein–Protein Interaction" @default.
• W2064308921 cites W1846318147 @default.
• W2064308921 cites W1985912212 @default.
• W2064308921 cites W1989527063 @default.
• W2064308921 cites W1993575340 @default.
• W2064308921 cites W2002112906 @default.
• W2064308921 cites W2002183278 @default.
• W2064308921 cites W2008284182 @default.
• W2064308921 cites W2019174587 @default.
• W2064308921 cites W2026370952 @default.
• W2064308921 cites W2031011895 @default.
• W2064308921 cites W2044682925 @default.
• W2064308921 cites W2052698568 @default.
• W2064308921 cites W2057520802 @default.
• W2064308921 cites W2058527555 @default.
• W2064308921 cites W2059508494 @default.
• W2064308921 cites W2065625685 @default.
• W2064308921 cites W2065654184 @default.
• W2064308921 cites W2073379847 @default.
• W2064308921 cites W2073939564 @default.
• W2064308921 cites W2090991396 @default.
• W2064308921 cites W2092079812 @default.
• W2064308921 cites W2106522450 @default.
• W2064308921 cites W2121433760 @default.
• W2064308921 cites W2125823078 @default.
• W2064308921 cites W2129475622 @default.
• W2064308921 cites W2150192011 @default.
• W2064308921 cites W2151546364 @default.
• W2064308921 cites W2153133904 @default.
• W2064308921 cites W2162820914 @default.
• W2064308921 cites W2175687390 @default.
• W2064308921 doi "https://doi.org/10.1016/j.jmb.2011.06.009" @default.
• W2064308921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3143254" @default.
• W2064308921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21689661" @default.
• W2064308921 hasPublicationYear "2011" @default.
• W2064308921 type Work @default.
• W2064308921 sameAs 2064308921 @default.
• W2064308921 citedByCount "7" @default.
• W2064308921 countsByYear W20643089212012 @default.
• W2064308921 countsByYear W20643089212013 @default.
• W2064308921 countsByYear W20643089212014 @default.
• W2064308921 countsByYear W20643089212018 @default.
• W2064308921 crossrefType "journal-article" @default.
• W2064308921 hasAuthorship W2064308921A5015133421 @default.
• W2064308921 hasAuthorship W2064308921A5037693404 @default.
• W2064308921 hasAuthorship W2064308921A5046287489 @default.
• W2064308921 hasAuthorship W2064308921A5057683371 @default.
• W2064308921 hasAuthorship W2064308921A5060558357 @default.
• W2064308921 hasAuthorship W2064308921A5066594495 @default.
• W2064308921 hasAuthorship W2064308921A5079686174 @default.
• W2064308921 hasAuthorship W2064308921A5084369008 @default.
• W2064308921 hasAuthorship W2064308921A5088135588 @default.
• W2064308921 hasBestOaLocation W20643089212 @default.
• W2064308921 hasConcept C104317684 @default.
• W2064308921 hasConcept C143065580 @default.
• W2064308921 hasConcept C147816474 @default.
• W2064308921 hasConcept C152769699 @default.
• W2064308921 hasConcept C16318435 @default.
• W2064308921 hasConcept C181199279 @default.
• W2064308921 hasConcept C185592680 @default.
• W2064308921 hasConcept C199360897 @default.
• W2064308921 hasConcept C41008148 @default.
• W2064308921 hasConcept C47701112 @default.
• W2064308921 hasConcept C53942344 @default.
• W2064308921 hasConcept C55493867 @default.
• W2064308921 hasConcept C70721500 @default.
• W2064308921 hasConcept C86803240 @default.
• W2064308921 hasConceptScore W2064308921C104317684 @default.
• W2064308921 hasConceptScore W2064308921C143065580 @default.
• W2064308921 hasConceptScore W2064308921C147816474 @default.
• W2064308921 hasConceptScore W2064308921C152769699 @default.
• W2064308921 hasConceptScore W2064308921C16318435 @default.
• W2064308921 hasConceptScore W2064308921C181199279 @default.
• W2064308921 hasConceptScore W2064308921C185592680 @default.
• W2064308921 hasConceptScore W2064308921C199360897 @default.
• W2064308921 hasConceptScore W2064308921C41008148 @default.
• W2064308921 hasConceptScore W2064308921C47701112 @default.
• W2064308921 hasConceptScore W2064308921C53942344 @default.
• W2064308921 hasConceptScore W2064308921C55493867 @default.
• W2064308921 hasConceptScore W2064308921C70721500 @default.
• W2064308921 hasConceptScore W2064308921C86803240 @default.
• W2064308921 hasIssue "2" @default.
• W2064308921 hasLocation W20643089211 @default.
• W2064308921 hasLocation W20643089212 @default.
• W2064308921 hasLocation W20643089213 @default.

• next