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- W2064346062 abstract "Isolated tick salivary glands, permeabilized with digitonin in the presence of the Ca2+ uptake inhibitors, sodium azide and vanadate, released Ca2+ in response to 20 μM inositol-1,4,5-trisphosphate (IP3). Inositol-1-phosphate (IP1) and inositol-1,4-bisphosphate (IP2) appeared to stimulate an uptake of Ca2+ into whole glands. Inositol-1,4,5-trisphosphate caused release of Ca2+ from a 100,000 g microsome enriched pellet; however, IP1 and IP2 were ineffective in stimulating an uptake or efflux of Ca2+. The combined 900 and 11,500 g pellets showed no significant release of Ca2+ in response to addition of IP3. Inositol-1,4,5-trisphosphate concentrations as low as 1 μM are capable of stimulating a significant release of Ca2+ from microsomes. Results suggest that intracellular Ca2+ is mobilized from microsomal intracellular stores in response to agonists which increase cytosolic IP3 in tick salivary glands. Results also suggest a possible role for IP1 and IP2 or both in stimulating an uptake of Ca2+ into vanadate and azide-insensitive intracellular pools." @default.
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- W2064346062 date "1990-01-01" @default.
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- W2064346062 title "The role of inositol 1,4,5-trisphosphate in mobilizing calcium from intracellular stores in the salivary glands of Amblyomma americanum (L.)" @default.
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