Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064396442> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2064396442 endingPage "500" @default.
- W2064396442 startingPage "495" @default.
- W2064396442 abstract "Recently, we demonstrated that the N-terminal region of mouse α-dystroglycan represents an autonomously folding globular domain, organized into at least two subdomains (Brancaccio et al., Eur. J. Biochem. 246, 166–172, 1997). We have now found a similarity between a part of the α-dystroglycan N-terminal sequence (approximately from position 80 to 180) and several protein sequences belonging to the immunoglobulin κ family. Moreover, we have recombinantly expressed and purified a 31 kDa protein fragment which matches the entire α-dystroglycan N-terminal globular domain. To prevent the action of bacterial endogenous proteases and/or thrombin, which cleaves the protein into two fragments at an Arg-Ala trypsin-sensitive site in positions 168–169, we have introduced a single mutation (Arg168 → His), thus making the whole domain more stable and suitable for crystallization. Crystals of this mutant protein were obtained by vapor diffusion using the hanging drop technique, and they diffract to 0.28 nm Bragg spacing." @default.
- W2064396442 created "2016-06-24" @default.
- W2064396442 creator A5006266659 @default.
- W2064396442 creator A5012954903 @default.
- W2064396442 creator A5089649845 @default.
- W2064396442 date "1998-11-01" @default.
- W2064396442 modified "2023-10-14" @default.
- W2064396442 title "Sequence analysis suggests the presence of an IG-like domain in the N-terminal region of α-dystroglycan which was crystallized after mutation of a protease susceptible site (Arg168 → His)" @default.
- W2064396442 cites W1968437028 @default.
- W2064396442 cites W1975085160 @default.
- W2064396442 cites W1979452378 @default.
- W2064396442 cites W1992338919 @default.
- W2064396442 cites W2008115339 @default.
- W2064396442 cites W2014694459 @default.
- W2064396442 cites W2027637471 @default.
- W2064396442 cites W2031209863 @default.
- W2064396442 cites W2031779604 @default.
- W2064396442 cites W2066114684 @default.
- W2064396442 cites W2078683141 @default.
- W2064396442 cites W2093489908 @default.
- W2064396442 cites W2127883250 @default.
- W2064396442 cites W2128561011 @default.
- W2064396442 cites W2148269129 @default.
- W2064396442 cites W2463771178 @default.
- W2064396442 doi "https://doi.org/10.1016/s0945-053x(98)90097-x" @default.
- W2064396442 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9881601" @default.
- W2064396442 hasPublicationYear "1998" @default.
- W2064396442 type Work @default.
- W2064396442 sameAs 2064396442 @default.
- W2064396442 citedByCount "16" @default.
- W2064396442 countsByYear W20643964422019 @default.
- W2064396442 crossrefType "journal-article" @default.
- W2064396442 hasAuthorship W2064396442A5006266659 @default.
- W2064396442 hasAuthorship W2064396442A5012954903 @default.
- W2064396442 hasAuthorship W2064396442A5089649845 @default.
- W2064396442 hasConcept C104317684 @default.
- W2064396442 hasConcept C143065580 @default.
- W2064396442 hasConcept C1491633281 @default.
- W2064396442 hasConcept C153911025 @default.
- W2064396442 hasConcept C181199279 @default.
- W2064396442 hasConcept C182220744 @default.
- W2064396442 hasConcept C185592680 @default.
- W2064396442 hasConcept C2776714187 @default.
- W2064396442 hasConcept C2778384963 @default.
- W2064396442 hasConcept C2779814568 @default.
- W2064396442 hasConcept C501734568 @default.
- W2064396442 hasConcept C55493867 @default.
- W2064396442 hasConcept C86803240 @default.
- W2064396442 hasConceptScore W2064396442C104317684 @default.
- W2064396442 hasConceptScore W2064396442C143065580 @default.
- W2064396442 hasConceptScore W2064396442C1491633281 @default.
- W2064396442 hasConceptScore W2064396442C153911025 @default.
- W2064396442 hasConceptScore W2064396442C181199279 @default.
- W2064396442 hasConceptScore W2064396442C182220744 @default.
- W2064396442 hasConceptScore W2064396442C185592680 @default.
- W2064396442 hasConceptScore W2064396442C2776714187 @default.
- W2064396442 hasConceptScore W2064396442C2778384963 @default.
- W2064396442 hasConceptScore W2064396442C2779814568 @default.
- W2064396442 hasConceptScore W2064396442C501734568 @default.
- W2064396442 hasConceptScore W2064396442C55493867 @default.
- W2064396442 hasConceptScore W2064396442C86803240 @default.
- W2064396442 hasFunder F4320320924 @default.
- W2064396442 hasIssue "7" @default.
- W2064396442 hasLocation W20643964421 @default.
- W2064396442 hasLocation W20643964422 @default.
- W2064396442 hasOpenAccess W2064396442 @default.
- W2064396442 hasPrimaryLocation W20643964421 @default.
- W2064396442 hasRelatedWork W1802364313 @default.
- W2064396442 hasRelatedWork W1983247216 @default.
- W2064396442 hasRelatedWork W2115254837 @default.
- W2064396442 hasRelatedWork W2143995628 @default.
- W2064396442 hasRelatedWork W2161390102 @default.
- W2064396442 hasRelatedWork W2182122486 @default.
- W2064396442 hasRelatedWork W2383040127 @default.
- W2064396442 hasRelatedWork W2405089661 @default.
- W2064396442 hasRelatedWork W2614341759 @default.
- W2064396442 hasRelatedWork W3082408526 @default.
- W2064396442 hasVolume "17" @default.
- W2064396442 isParatext "false" @default.
- W2064396442 isRetracted "false" @default.
- W2064396442 magId "2064396442" @default.
- W2064396442 workType "article" @default.