FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064396919> ?p ?o ?g. }

• W2064396919 endingPage "e1001278" @default.
• W2064396919 startingPage "e1001278" @default.
• W2064396919 abstract "Growing experimental evidence indicates that, in addition to the physical virion components, the non-structural proteins of hepatitis C virus (HCV) are intimately involved in orchestrating morphogenesis. Since it is dispensable for HCV RNA replication, the non-structural viral protein NS2 is suggested to play a central role in HCV particle assembly. However, despite genetic evidences, we have almost no understanding about NS2 protein-protein interactions and their role in the production of infectious particles. Here, we used co-immunoprecipitation and/or fluorescence resonance energy transfer with fluorescence lifetime imaging microscopy analyses to study the interactions between NS2 and the viroporin p7 and the HCV glycoprotein E2. In addition, we used alanine scanning insertion mutagenesis as well as other mutations in the context of an infectious virus to investigate the functional role of NS2 in HCV assembly. Finally, the subcellular localization of NS2 and several mutants was analyzed by confocal microscopy. Our data demonstrate molecular interactions between NS2 and p7 and E2. Furthermore, we show that, in the context of an infectious virus, NS2 accumulates over time in endoplasmic reticulum-derived dotted structures and colocalizes with both the envelope glycoproteins and components of the replication complex in close proximity to the HCV core protein and lipid droplets, a location that has been shown to be essential for virus assembly. We show that NS2 transmembrane region is crucial for both E2 interaction and subcellular localization. Moreover, specific mutations in core, envelope proteins, p7 and NS5A reported to abolish viral assembly changed the subcellular localization of NS2 protein. Together, these observations indicate that NS2 protein attracts the envelope proteins at the assembly site and it crosstalks with non-structural proteins for virus assembly." @default.
• W2064396919 created "2016-06-24" @default.
• W2064396919 creator A5024515740 @default.
• W2064396919 creator A5030252277 @default.
• W2064396919 creator A5037924047 @default.
• W2064396919 creator A5057771633 @default.
• W2064396919 creator A5066836024 @default.
• W2064396919 creator A5068576789 @default.
• W2064396919 creator A5073351964 @default.
• W2064396919 creator A5073926339 @default.
• W2064396919 creator A5086442553 @default.
• W2064396919 creator A5087727909 @default.
• W2064396919 creator A5091273319 @default.
• W2064396919 date "2011-02-10" @default.
• W2064396919 modified "2023-10-18" @default.
• W2064396919 title "NS2 Protein of Hepatitis C Virus Interacts with Structural and Non-Structural Proteins towards Virus Assembly" @default.
• W2064396919 cites W1542375433 @default.
• W2064396919 cites W1562770137 @default.
• W2064396919 cites W1635062911 @default.
• W2064396919 cites W1657414671 @default.
• W2064396919 cites W1933496969 @default.
• W2064396919 cites W1963825210 @default.
• W2064396919 cites W1967946955 @default.
• W2064396919 cites W1970324388 @default.
• W2064396919 cites W1971311328 @default.
• W2064396919 cites W1971505503 @default.
• W2064396919 cites W1976672938 @default.
• W2064396919 cites W1982315879 @default.
• W2064396919 cites W1984550703 @default.
• W2064396919 cites W1991947489 @default.
• W2064396919 cites W1993651229 @default.
• W2064396919 cites W2003579943 @default.
• W2064396919 cites W2009952289 @default.
• W2064396919 cites W2011220377 @default.
• W2064396919 cites W2017627871 @default.
• W2064396919 cites W2021199098 @default.
• W2064396919 cites W2022869504 @default.
• W2064396919 cites W2023645777 @default.
• W2064396919 cites W2029323175 @default.
• W2064396919 cites W2030441312 @default.
• W2064396919 cites W2030749742 @default.
• W2064396919 cites W2036725587 @default.
• W2064396919 cites W2041818968 @default.
• W2064396919 cites W2043825818 @default.
• W2064396919 cites W2044193462 @default.
• W2064396919 cites W2045571454 @default.
• W2064396919 cites W2049746784 @default.
• W2064396919 cites W2051812008 @default.
• W2064396919 cites W2057040842 @default.
• W2064396919 cites W2057667075 @default.
• W2064396919 cites W2057967679 @default.
• W2064396919 cites W2062395204 @default.
• W2064396919 cites W2062848152 @default.
• W2064396919 cites W2064304037 @default.
• W2064396919 cites W2068128206 @default.
• W2064396919 cites W2071136569 @default.
• W2064396919 cites W2075557556 @default.
• W2064396919 cites W2077458665 @default.
• W2064396919 cites W2080835548 @default.
• W2064396919 cites W2085061590 @default.
• W2064396919 cites W2095988962 @default.
• W2064396919 cites W2103438957 @default.
• W2064396919 cites W2108463852 @default.
• W2064396919 cites W2117697615 @default.
• W2064396919 cites W2124025387 @default.
• W2064396919 cites W2127444976 @default.
• W2064396919 cites W2128237394 @default.
• W2064396919 cites W2129995310 @default.
• W2064396919 cites W2135495604 @default.
• W2064396919 cites W2135537539 @default.
• W2064396919 cites W2143824349 @default.
• W2064396919 cites W2147126871 @default.
• W2064396919 cites W2147966537 @default.
• W2064396919 cites W2148910155 @default.
• W2064396919 cites W2150368001 @default.
• W2064396919 cites W2151581088 @default.
• W2064396919 cites W2151898297 @default.
• W2064396919 cites W2156004093 @default.
• W2064396919 cites W2161890443 @default.
• W2064396919 cites W2168745915 @default.
• W2064396919 cites W2502759836 @default.
• W2064396919 doi "https://doi.org/10.1371/journal.ppat.1001278" @default.
• W2064396919 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3037360" @default.
• W2064396919 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21347350" @default.
• W2064396919 hasPublicationYear "2011" @default.
• W2064396919 type Work @default.
• W2064396919 sameAs 2064396919 @default.
• W2064396919 citedByCount "145" @default.
• W2064396919 countsByYear W20643969192012 @default.
• W2064396919 countsByYear W20643969192013 @default.
• W2064396919 countsByYear W20643969192014 @default.
• W2064396919 countsByYear W20643969192015 @default.
• W2064396919 countsByYear W20643969192016 @default.
• W2064396919 countsByYear W20643969192017 @default.
• W2064396919 countsByYear W20643969192018 @default.
• W2064396919 countsByYear W20643969192019 @default.
• W2064396919 countsByYear W20643969192020 @default.
• W2064396919 countsByYear W20643969192021 @default.

• next