FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064407029> ?p ?o ?g. }

• W2064407029 endingPage "296" @default.
• W2064407029 startingPage "287" @default.
• W2064407029 abstract "In the companion article, we reported that the local phosphate (Pi) concentration triggers apoptosis in epiphyseal chondrocytes. The goal of the current investigation was to evaluate the apoptotic process in relationship to the energy status of cells in the growth plate. For these studies, we used sections of the adolescent growth plate, as well as cells isolated from the tissue. We found that there was a maturation-dependent loss of mitochondrial function in growth plate chondrocytes and these cells generated energy by glycolysis. Since treatment with the uncoupler 2,4-dinitrophenol as well as the site-specific inhibitors antimycin A and rotenone failed to elicit a further increase in the activity of the glycolytic pathway, we concluded that oxidative metabolism was minimum in these cells. Flow cytometric studies of growth plate cells and confocal microscopy of growth plate sections using the mitochondrial probes Rh123 and DiOC6(3) provided unequivocal evidence that there was loss of mitochondrial membrane potential in hypertrophic cells. Furthermore, the intrinsic fluorescence of the flavoprotein lipoamide dehydrogenase complex of the electron transport chain revealed that the mitochondria were in an oxidized state. Finally, we assessed Bcl-2 expression in these cells. Although immunohistochemical and Western blot analysis showed that the chick cells contained a low level of the anti-apoptotic protein Bcl-2, reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that transcripts were present in chondrocytes. Based on these observations, we suggest that terminally differentiated chondrocytes undergo a maturation-dependent loss of mitochondrial function. In concert with the low expression of Bcl-2, they become sensitive to signals for programmed cell death. We hypothesize that Pi triggers apoptosis in these energy-compromised cells by promoting a mitochondrial membrane transition, thereby inducing the death process. J. Cell. Physiol. 179:287–296, 1999. © 1999 Wiley-Liss, Inc." @default.
• W2064407029 created "2016-06-24" @default.
• W2064407029 creator A5005273452 @default.
• W2064407029 creator A5010174535 @default.
• W2064407029 creator A5019521528 @default.
• W2064407029 creator A5025149114 @default.
• W2064407029 creator A5059642352 @default.
• W2064407029 date "1999-06-01" @default.
• W2064407029 modified "2023-10-18" @default.
• W2064407029 title "Chondrocyte death is linked to development of a mitochondrial membrane permeability transition in the growth plate" @default.
• W2064407029 cites W146098328 @default.
• W2064407029 cites W1513518426 @default.
• W2064407029 cites W1567258255 @default.
• W2064407029 cites W1964519636 @default.
• W2064407029 cites W1966539065 @default.
• W2064407029 cites W1980801451 @default.
• W2064407029 cites W1981108341 @default.
• W2064407029 cites W1987605489 @default.
• W2064407029 cites W1992190124 @default.
• W2064407029 cites W1994577132 @default.
• W2064407029 cites W2007904889 @default.
• W2064407029 cites W2008563659 @default.
• W2064407029 cites W2009549355 @default.
• W2064407029 cites W2015981602 @default.
• W2064407029 cites W2022927403 @default.
• W2064407029 cites W2039220157 @default.
• W2064407029 cites W2053918739 @default.
• W2064407029 cites W2055431823 @default.
• W2064407029 cites W2064182974 @default.
• W2064407029 cites W2065151410 @default.
• W2064407029 cites W2068851063 @default.
• W2064407029 cites W2071532689 @default.
• W2064407029 cites W2079903643 @default.
• W2064407029 cites W2084417037 @default.
• W2064407029 cites W2095924030 @default.
• W2064407029 cites W2098712005 @default.
• W2064407029 cites W2099931271 @default.
• W2064407029 cites W2103461739 @default.
• W2064407029 cites W2108131089 @default.
• W2064407029 cites W2115195316 @default.
• W2064407029 cites W2219120320 @default.
• W2064407029 cites W39614084 @default.
• W2064407029 cites W4236608613 @default.
• W2064407029 doi "https://doi.org/10.1002/(sici)1097-4652(199906)179:3<287::aid-jcp6>3.0.co;2-t" @default.
• W2064407029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10228947" @default.
• W2064407029 hasPublicationYear "1999" @default.
• W2064407029 type Work @default.
• W2064407029 sameAs 2064407029 @default.
• W2064407029 citedByCount "45" @default.
• W2064407029 countsByYear W20644070292012 @default.
• W2064407029 countsByYear W20644070292013 @default.
• W2064407029 countsByYear W20644070292015 @default.
• W2064407029 countsByYear W20644070292016 @default.
• W2064407029 countsByYear W20644070292017 @default.
• W2064407029 countsByYear W20644070292019 @default.
• W2064407029 countsByYear W20644070292020 @default.
• W2064407029 countsByYear W20644070292021 @default.
• W2064407029 countsByYear W20644070292023 @default.
• W2064407029 crossrefType "journal-article" @default.
• W2064407029 hasAuthorship W2064407029A5005273452 @default.
• W2064407029 hasAuthorship W2064407029A5010174535 @default.
• W2064407029 hasAuthorship W2064407029A5019521528 @default.
• W2064407029 hasAuthorship W2064407029A5025149114 @default.
• W2064407029 hasAuthorship W2064407029A5059642352 @default.
• W2064407029 hasConcept C13591479 @default.
• W2064407029 hasConcept C181911157 @default.
• W2064407029 hasConcept C190283241 @default.
• W2064407029 hasConcept C202751555 @default.
• W2064407029 hasConcept C2779965811 @default.
• W2064407029 hasConcept C2781403057 @default.
• W2064407029 hasConcept C28859421 @default.
• W2064407029 hasConcept C31573885 @default.
• W2064407029 hasConcept C55493867 @default.
• W2064407029 hasConcept C57600042 @default.
• W2064407029 hasConcept C86803240 @default.
• W2064407029 hasConcept C95444343 @default.
• W2064407029 hasConceptScore W2064407029C13591479 @default.
• W2064407029 hasConceptScore W2064407029C181911157 @default.
• W2064407029 hasConceptScore W2064407029C190283241 @default.
• W2064407029 hasConceptScore W2064407029C202751555 @default.
• W2064407029 hasConceptScore W2064407029C2779965811 @default.
• W2064407029 hasConceptScore W2064407029C2781403057 @default.
• W2064407029 hasConceptScore W2064407029C28859421 @default.
• W2064407029 hasConceptScore W2064407029C31573885 @default.
• W2064407029 hasConceptScore W2064407029C55493867 @default.
• W2064407029 hasConceptScore W2064407029C57600042 @default.
• W2064407029 hasConceptScore W2064407029C86803240 @default.
• W2064407029 hasConceptScore W2064407029C95444343 @default.
• W2064407029 hasIssue "3" @default.
• W2064407029 hasLocation W20644070291 @default.
• W2064407029 hasLocation W20644070292 @default.
• W2064407029 hasOpenAccess W2064407029 @default.
• W2064407029 hasPrimaryLocation W20644070291 @default.
• W2064407029 hasRelatedWork W2063099262 @default.
• W2064407029 hasRelatedWork W2071925317 @default.
• W2064407029 hasRelatedWork W2116199074 @default.
• W2064407029 hasRelatedWork W2348771880 @default.
• W2064407029 hasRelatedWork W2366206075 @default.

• next