FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064470249> ?p ?o ?g. }

• W2064470249 endingPage "175" @default.
• W2064470249 startingPage "165" @default.
• W2064470249 abstract "While emphasis has been placed upon those proteins which either mediate or respond to the rapid influx of calcium following depolarization, there has been little emphasis upon those proteins which aid in the reequilibration of the membrane potential. In an effort to identify presynaptic membrane proteins implicated in neurosecretion, monoclonal antibodies were screened against proteins which cosegregated with neuronal voltage-dependent calcium channels (VDCC) following immunoprecipitation. One monoclonal antibody (mAb 9A7) identified a 110-kDa protein. Micropeptide sequencing of (i) the mAb 9A7 immunoaffinity purified antigen and (ii) the 110-kDa protein present in the neuronal (N-type) VDCC preparation (McEneryet al.,1991,Proc. Natl. Acad. Sci.88, 11095–11099) indicated identity with the alpha subunit(s) of the Na,K-ATPase. Further characterization by Western blotting, immunochemical localization, and immunoaffinity purification indicated that mAb 9A7 not only recognized the alpha3 isoform which is predominant in neuronal tissues but also identified the alpha1 and alpha2 isoforms. mAb 9A7 exhibited a wide cross-species reactivity and recognized human, rat, and mouse alpha subunit isoforms at an internal epitope. The pan-specificity of mAb 9A7 and the differential mobility of the alpha1 isoform relative to the alpha2 and alpha3 permitted parallel detection of multiple alpha isoforms. Western blot analysis of undifferentiated rat pheochromocytoma cell line (PC12) and human neuroblastoma (IMR32) cells indicated coexpression of the alpha1 and alpha3 isozymes. Upon differentiation of IMR32 cells by dibutrylyl-cAMP, a substantial increase in the alpha3 relative to the alpha1 isoform was observed. While the enrichment of total Na,K-ATPase may reflect the increased demand for ATP-dependent ion transport as IMR32 cells become more excitable, the specific increase in the alpha3 isoform suggests a unique role of this isoform during IMR32 cell differentiation." @default.
• W2064470249 created "2016-06-24" @default.
• W2064470249 creator A5009729065 @default.
• W2064470249 creator A5016493447 @default.
• W2064470249 creator A5043280562 @default.
• W2064470249 creator A5050546759 @default.
• W2064470249 creator A5066560112 @default.
• W2064470249 creator A5066675811 @default.
• W2064470249 creator A5067844076 @default.
• W2064470249 creator A5074809730 @default.
• W2064470249 date "1997-08-01" @default.
• W2064470249 modified "2023-10-18" @default.
• W2064470249 title "Parallel Detection of Na,K-ATPase Alpha Subunit Isoforms by Pan-Specific Monoclonal mAb 9A7" @default.
• W2064470249 cites W1486133777 @default.
• W2064470249 cites W1490238549 @default.
• W2064470249 cites W1511107734 @default.
• W2064470249 cites W1520012696 @default.
• W2064470249 cites W1522720205 @default.
• W2064470249 cites W1525632749 @default.
• W2064470249 cites W1531907208 @default.
• W2064470249 cites W1542496508 @default.
• W2064470249 cites W1551967741 @default.
• W2064470249 cites W1562290896 @default.
• W2064470249 cites W1574239487 @default.
• W2064470249 cites W1588451507 @default.
• W2064470249 cites W1598566568 @default.
• W2064470249 cites W1604450112 @default.
• W2064470249 cites W1867426858 @default.
• W2064470249 cites W1965102714 @default.
• W2064470249 cites W1967705425 @default.
• W2064470249 cites W1971884355 @default.
• W2064470249 cites W1978680594 @default.
• W2064470249 cites W1981283691 @default.
• W2064470249 cites W1984829055 @default.
• W2064470249 cites W1986849002 @default.
• W2064470249 cites W1991640772 @default.
• W2064470249 cites W1994451390 @default.
• W2064470249 cites W2005114186 @default.
• W2064470249 cites W2017654726 @default.
• W2064470249 cites W2019712500 @default.
• W2064470249 cites W2020300164 @default.
• W2064470249 cites W2032623967 @default.
• W2064470249 cites W2042011830 @default.
• W2064470249 cites W2043577188 @default.
• W2064470249 cites W2048706584 @default.
• W2064470249 cites W2051853796 @default.
• W2064470249 cites W2056254399 @default.
• W2064470249 cites W2057255195 @default.
• W2064470249 cites W2063255326 @default.
• W2064470249 cites W2066605884 @default.
• W2064470249 cites W2068365122 @default.
• W2064470249 cites W2075607213 @default.
• W2064470249 cites W2083621243 @default.
• W2064470249 cites W2089719366 @default.
• W2064470249 cites W2094596294 @default.
• W2064470249 cites W2100837269 @default.
• W2064470249 cites W2103316668 @default.
• W2064470249 cites W2107540632 @default.
• W2064470249 cites W2110565876 @default.
• W2064470249 cites W2110817851 @default.
• W2064470249 cites W2111807682 @default.
• W2064470249 cites W2116672207 @default.
• W2064470249 cites W2121913898 @default.
• W2064470249 cites W2122284031 @default.
• W2064470249 cites W2130425395 @default.
• W2064470249 cites W2133280120 @default.
• W2064470249 cites W2144157590 @default.
• W2064470249 cites W2145308307 @default.
• W2064470249 cites W2146329839 @default.
• W2064470249 cites W2153794151 @default.
• W2064470249 cites W2161677604 @default.
• W2064470249 cites W2274003284 @default.
• W2064470249 cites W2341150623 @default.
• W2064470249 cites W237578561 @default.
• W2064470249 cites W2402434012 @default.
• W2064470249 cites W2411668369 @default.
• W2064470249 cites W4232534952 @default.
• W2064470249 doi "https://doi.org/10.1006/abbi.1997.0183" @default.
• W2064470249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9244394" @default.
• W2064470249 hasPublicationYear "1997" @default.
• W2064470249 type Work @default.
• W2064470249 sameAs 2064470249 @default.
• W2064470249 citedByCount "20" @default.
• W2064470249 countsByYear W20644702492012 @default.
• W2064470249 countsByYear W20644702492017 @default.
• W2064470249 crossrefType "journal-article" @default.
• W2064470249 hasAuthorship W2064470249A5009729065 @default.
• W2064470249 hasAuthorship W2064470249A5016493447 @default.
• W2064470249 hasAuthorship W2064470249A5043280562 @default.
• W2064470249 hasAuthorship W2064470249A5050546759 @default.
• W2064470249 hasAuthorship W2064470249A5066560112 @default.
• W2064470249 hasAuthorship W2064470249A5066675811 @default.
• W2064470249 hasAuthorship W2064470249A5067844076 @default.
• W2064470249 hasAuthorship W2064470249A5074809730 @default.
• W2064470249 hasConcept C104292427 @default.
• W2064470249 hasConcept C104317684 @default.
• W2064470249 hasConcept C119795356 @default.
• W2064470249 hasConcept C153911025 @default.

• next