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• W2064503919 endingPage "510" @default.
• W2064503919 startingPage "501" @default.
• W2064503919 abstract "SUMMARY Green fluorescent protein (GFP)-parasite transfectants have been widely used as a tool for studying disease pathogenesis in several protozoan models and their application in drug screening assays has increased rapidly. In the past decade, the expression of GFP has been established in several Leishmania species, mostly for in vitro studies. The current work reports generation of four transgenic parasites constitutively expressing GFP ( Leishmania mexicana, Leishmania aethiopica, Leishmania tropica and Leishmania major ) and their validation as a representative model of infection. This is the first report where stable expression of GFP has been achieved in L. aethiopica and L. tropica . Integration of GFP was accomplished through homologous recombination of the expression construct, pRib1.2 α NEO α GFP downstream of the 18S rRNA promoter in all species. A homogeneous and high level expression of GFP was detected in both the promastigote and the intracellular amastigote stages. All transgenic species showed the same growth pattern, ability to infect mammalian host cells and sensitivity to reference drugs as their wild type counterparts. All four transgenic Leishmania are confirmed as models for in vitro and possibly in vivo infections and represent an ideal tool for medium throughput testing of compound libraries." @default.
• W2064503919 created "2016-06-24" @default.
• W2064503919 creator A5047279701 @default.
• W2064503919 creator A5071066438 @default.
• W2064503919 creator A5087200874 @default.
• W2064503919 date "2013-11-20" @default.
• W2064503919 modified "2023-10-18" @default.
• W2064503919 title "Development and validation of four<i>Leishmania</i>species constitutively expressing GFP protein. A model for drug discovery and disease pathogenesis studies" @default.
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• W2064503919 doi "https://doi.org/10.1017/s0031182013001777" @default.
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• W2064503919 hasPublicationYear "2013" @default.
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