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• W2064508959 abstract "The patatin-like phospholipase domain containing 3 (PNPLA3, also called adiponutrin, ADPN) is a membrane-bound protein highly expressed in the liver. The genetic variant I148M (rs738409) was found to be associated with progression of chronic liver disease. We aimed to establish a protein purification protocol in a yeast system (Pichia pastoris) and to examine the human PNPLA3 enzymatic activity, substrate specificity and the I148M mutation effect. hPNPLA3 148I wild type and 148M mutant cDNA were cloned into P. pastoris expression vectors. Yeast cells were grown in 3L fermentors. PNPLA3 protein was purified from membrane fractions by Ni-affinity chromatography. Enzymatic activity was assessed using radiolabeled substrates. Both 148I wild type and 148M mutant proteins are localized to the membrane. The wild type protein shows a predominant lipase activity with mild lysophosphatidic acid acyl transferase activity (LPAAT) and the I148M mutation results in a loss of function of both these activities. Our data show that PNPLA3 has a predominant lipase activity and I148M mutation results in a loss of function." @default.
• W2064508959 created "2016-06-24" @default.
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• W2064508959 date "2014-04-01" @default.
• W2064508959 modified "2023-10-18" @default.
• W2064508959 title "Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function" @default.
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• W2064508959 doi "https://doi.org/10.1016/j.bbalip.2013.12.006" @default.
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