FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064549818> ?p ?o ?g. }

• W2064549818 endingPage "154" @default.
• W2064549818 startingPage "141" @default.
• W2064549818 abstract "Testosterone supplementation in men decreases fat mass; however, the mechanisms by which it inhibits fat mass are unknown. We hypothesized that testosterone inhibits adipogenic differentiation of preadipocytes by activation of androgen receptor (AR)/β-catenin interaction and subsequent translocation of this complex to the nucleus thereby bypassing canonical Wnt signaling. We tested this hypothesis in 3T3-L1 cells that differentiate to form fat cells in adipogenic medium. We found that these cells express AR and that testosterone and dihydrotestosterone dose-dependently inhibited adipogenic differentiation as analyzed by Oil Red O staining and down-regulation of CCAAT/enhancer binding protein-α and -δ and peroxisome proliferator-activated receptor-γ2 protein and mRNA. These inhibitory effects of androgens were partially blocked by flutamide or bicalutamide. Androgen treatment was associated with nuclear translocation of β-catenin and AR. Immunoprecipitation studies demonstrated association of β-catenin with AR and T-cell factor 4 (TCF4) in the presence of androgens. Transfection of TCF4 cDNA inhibited adipogenic differentiation, whereas a dominant negative TCF4 cDNA construct induced adipogenesis and blocked testosterone’s inhibitory effects. Our gene array analysis indicates that testosterone treatment led to activation of some Wnt target genes. Expression of constitutively activated AR fused with VP-16 did not inhibit the expression of CCAAT/enhancer binding protein-α in the absence of androgens. Testosterone and dihydrotestosterone inhibit adipocyte differentiation in vitro through an AR-mediated nuclear translocation of β-catenin and activation of downstream Wnt signaling. These data provide evidence for a regulatory role for androgens in inhibiting adipogenic differentiation and a mechanistic explanation consistent with the observed reduction in fat mass in men treated with androgens." @default.
• W2064549818 created "2016-06-24" @default.
• W2064549818 creator A5025495625 @default.
• W2064549818 creator A5034784511 @default.
• W2064549818 creator A5041817081 @default.
• W2064549818 creator A5060750742 @default.
• W2064549818 creator A5069145942 @default.
• W2064549818 creator A5070055705 @default.
• W2064549818 creator A5071176339 @default.
• W2064549818 date "2006-01-01" @default.
• W2064549818 modified "2023-10-14" @default.
• W2064549818 title "Testosterone Inhibits Adipogenic Differentiation in 3T3-L1 Cells: Nuclear Translocation of Androgen Receptor Complex with β-Catenin and T-Cell Factor 4 May Bypass Canonical Wnt Signaling to Down-Regulate Adipogenic Transcription Factors" @default.
• W2064549818 cites W1536067283 @default.
• W2064549818 cites W1554762746 @default.
• W2064549818 cites W1907219612 @default.
• W2064549818 cites W1970919014 @default.
• W2064549818 cites W1975079427 @default.
• W2064549818 cites W1975853332 @default.
• W2064549818 cites W1976404585 @default.
• W2064549818 cites W1981766282 @default.
• W2064549818 cites W2008295589 @default.
• W2064549818 cites W2022810442 @default.
• W2064549818 cites W2027586930 @default.
• W2064549818 cites W2028122319 @default.
• W2064549818 cites W2029094183 @default.
• W2064549818 cites W2033728702 @default.
• W2064549818 cites W2043129312 @default.
• W2064549818 cites W2053083708 @default.
• W2064549818 cites W2061144747 @default.
• W2064549818 cites W2066707764 @default.
• W2064549818 cites W2068483959 @default.
• W2064549818 cites W2073209871 @default.
• W2064549818 cites W2073998126 @default.
• W2064549818 cites W2076764916 @default.
• W2064549818 cites W2079819294 @default.
• W2064549818 cites W2083403745 @default.
• W2064549818 cites W2084148944 @default.
• W2064549818 cites W2086387074 @default.
• W2064549818 cites W2097914285 @default.
• W2064549818 cites W2097959454 @default.
• W2064549818 cites W2102076625 @default.
• W2064549818 cites W2106680203 @default.
• W2064549818 cites W2108727531 @default.
• W2064549818 cites W2109494488 @default.
• W2064549818 cites W2111369394 @default.
• W2064549818 cites W2117231350 @default.
• W2064549818 cites W2121643683 @default.
• W2064549818 cites W2128400771 @default.
• W2064549818 cites W2129627391 @default.
• W2064549818 cites W2132485471 @default.
• W2064549818 cites W2134710844 @default.
• W2064549818 cites W2143844562 @default.
• W2064549818 cites W2148294117 @default.
• W2064549818 cites W2155896219 @default.
• W2064549818 cites W2157452165 @default.
• W2064549818 cites W2160563411 @default.
• W2064549818 cites W2165011473 @default.
• W2064549818 cites W2170650848 @default.
• W2064549818 cites W2172256092 @default.
• W2064549818 cites W2173681478 @default.
• W2064549818 cites W2176176727 @default.
• W2064549818 cites W2239090104 @default.
• W2064549818 cites W56929786 @default.
• W2064549818 doi "https://doi.org/10.1210/en.2004-1649" @default.
• W2064549818 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4417624" @default.
• W2064549818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16210377" @default.
• W2064549818 hasPublicationYear "2006" @default.
• W2064549818 type Work @default.
• W2064549818 sameAs 2064549818 @default.
• W2064549818 citedByCount "332" @default.
• W2064549818 countsByYear W20645498182012 @default.
• W2064549818 countsByYear W20645498182013 @default.
• W2064549818 countsByYear W20645498182014 @default.
• W2064549818 countsByYear W20645498182015 @default.
• W2064549818 countsByYear W20645498182016 @default.
• W2064549818 countsByYear W20645498182017 @default.
• W2064549818 countsByYear W20645498182018 @default.
• W2064549818 countsByYear W20645498182019 @default.
• W2064549818 countsByYear W20645498182020 @default.
• W2064549818 countsByYear W20645498182021 @default.
• W2064549818 countsByYear W20645498182022 @default.
• W2064549818 countsByYear W20645498182023 @default.
• W2064549818 crossrefType "journal-article" @default.
• W2064549818 hasAuthorship W2064549818A5025495625 @default.
• W2064549818 hasAuthorship W2064549818A5034784511 @default.
• W2064549818 hasAuthorship W2064549818A5041817081 @default.
• W2064549818 hasAuthorship W2064549818A5060750742 @default.
• W2064549818 hasAuthorship W2064549818A5069145942 @default.
• W2064549818 hasAuthorship W2064549818A5070055705 @default.
• W2064549818 hasAuthorship W2064549818A5071176339 @default.
• W2064549818 hasBestOaLocation W20645498181 @default.
• W2064549818 hasConcept C121608353 @default.
• W2064549818 hasConcept C122927707 @default.
• W2064549818 hasConcept C126322002 @default.
• W2064549818 hasConcept C134018914 @default.
• W2064549818 hasConcept C137620995 @default.
• W2064549818 hasConcept C171089720 @default.
• W2064549818 hasConcept C185592680 @default.

• next