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- W2064554375 abstract "This paper describes a simple and stable staining reaction of inorganic phosphate applied to starch gels. During the reaction two blue redox compounds are formed, the reduction product of phosphoammonium-molybdate and the oxidation product of tetrabase. Tetrabase is a noncarcinogenic compound alternative to benzidine. Zymograms obtained by the present Pi procedure compared favorably with those obtained by reaction of the alcoholic part of an organic phosphate ester when AcP1 served as a test system. The results suggest that, when appropriate electrophoretic conditions have been worked out, the procedure may be extended to other enzymes, and possibly, to phosphohydrolases in general." @default.
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- W2064554375 date "1975-05-01" @default.
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- W2064554375 title "Phosphohydrolases: Inorganic phosphate reaction giving stably stained zymograms using an alternative to benzidine" @default.
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- W2064554375 doi "https://doi.org/10.1016/0003-2697(75)90548-5" @default.
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