Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064570845> ?p ?o ?g. }
- W2064570845 endingPage "2256" @default.
- W2064570845 startingPage "2241" @default.
- W2064570845 abstract "Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K m of 0.87 mg/ml at pH 10.0 and 60 °C. The enzyme is stable in the pH range of 8.0–10.0 and temperature ≤40 °C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture." @default.
- W2064570845 created "2016-06-24" @default.
- W2064570845 creator A5006657942 @default.
- W2064570845 creator A5007134556 @default.
- W2064570845 creator A5019740820 @default.
- W2064570845 creator A5020656426 @default.
- W2064570845 creator A5029417410 @default.
- W2064570845 creator A5040323824 @default.
- W2064570845 creator A5052135530 @default.
- W2064570845 creator A5069768683 @default.
- W2064570845 date "2012-06-14" @default.
- W2064570845 modified "2023-10-18" @default.
- W2064570845 title "Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance" @default.
- W2064570845 cites W1775749144 @default.
- W2064570845 cites W1965039627 @default.
- W2064570845 cites W1978584284 @default.
- W2064570845 cites W1980409685 @default.
- W2064570845 cites W2000935824 @default.
- W2064570845 cites W2001636985 @default.
- W2064570845 cites W2013452190 @default.
- W2064570845 cites W2014294063 @default.
- W2064570845 cites W2017198161 @default.
- W2064570845 cites W2030394993 @default.
- W2064570845 cites W2037407617 @default.
- W2064570845 cites W2045994310 @default.
- W2064570845 cites W2050711442 @default.
- W2064570845 cites W2051504504 @default.
- W2064570845 cites W2053832307 @default.
- W2064570845 cites W2056369560 @default.
- W2064570845 cites W2060314196 @default.
- W2064570845 cites W2062853176 @default.
- W2064570845 cites W2064836772 @default.
- W2064570845 cites W2076483202 @default.
- W2064570845 cites W2079190715 @default.
- W2064570845 cites W2088559313 @default.
- W2064570845 cites W2089731265 @default.
- W2064570845 cites W2096145490 @default.
- W2064570845 cites W2100837269 @default.
- W2064570845 cites W2126098557 @default.
- W2064570845 cites W2137583917 @default.
- W2064570845 cites W2145991107 @default.
- W2064570845 cites W2153195262 @default.
- W2064570845 cites W2157774942 @default.
- W2064570845 doi "https://doi.org/10.1007/s12010-012-9758-9" @default.
- W2064570845 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22695924" @default.
- W2064570845 hasPublicationYear "2012" @default.
- W2064570845 type Work @default.
- W2064570845 sameAs 2064570845 @default.
- W2064570845 citedByCount "24" @default.
- W2064570845 countsByYear W20645708452013 @default.
- W2064570845 countsByYear W20645708452014 @default.
- W2064570845 countsByYear W20645708452015 @default.
- W2064570845 countsByYear W20645708452016 @default.
- W2064570845 countsByYear W20645708452017 @default.
- W2064570845 countsByYear W20645708452018 @default.
- W2064570845 countsByYear W20645708452019 @default.
- W2064570845 countsByYear W20645708452020 @default.
- W2064570845 countsByYear W20645708452021 @default.
- W2064570845 countsByYear W20645708452022 @default.
- W2064570845 countsByYear W20645708452023 @default.
- W2064570845 crossrefType "journal-article" @default.
- W2064570845 hasAuthorship W2064570845A5006657942 @default.
- W2064570845 hasAuthorship W2064570845A5007134556 @default.
- W2064570845 hasAuthorship W2064570845A5019740820 @default.
- W2064570845 hasAuthorship W2064570845A5020656426 @default.
- W2064570845 hasAuthorship W2064570845A5029417410 @default.
- W2064570845 hasAuthorship W2064570845A5040323824 @default.
- W2064570845 hasAuthorship W2064570845A5052135530 @default.
- W2064570845 hasAuthorship W2064570845A5069768683 @default.
- W2064570845 hasBestOaLocation W20645708452 @default.
- W2064570845 hasConcept C101660505 @default.
- W2064570845 hasConcept C116084860 @default.
- W2064570845 hasConcept C163994747 @default.
- W2064570845 hasConcept C172854840 @default.
- W2064570845 hasConcept C181199279 @default.
- W2064570845 hasConcept C185592680 @default.
- W2064570845 hasConcept C193363816 @default.
- W2064570845 hasConcept C2776681119 @default.
- W2064570845 hasConcept C2777191336 @default.
- W2064570845 hasConcept C2778913249 @default.
- W2064570845 hasConcept C2780756937 @default.
- W2064570845 hasConcept C43617362 @default.
- W2064570845 hasConcept C55493867 @default.
- W2064570845 hasConcept C58121356 @default.
- W2064570845 hasConceptScore W2064570845C101660505 @default.
- W2064570845 hasConceptScore W2064570845C116084860 @default.
- W2064570845 hasConceptScore W2064570845C163994747 @default.
- W2064570845 hasConceptScore W2064570845C172854840 @default.
- W2064570845 hasConceptScore W2064570845C181199279 @default.
- W2064570845 hasConceptScore W2064570845C185592680 @default.
- W2064570845 hasConceptScore W2064570845C193363816 @default.
- W2064570845 hasConceptScore W2064570845C2776681119 @default.
- W2064570845 hasConceptScore W2064570845C2777191336 @default.
- W2064570845 hasConceptScore W2064570845C2778913249 @default.
- W2064570845 hasConceptScore W2064570845C2780756937 @default.
- W2064570845 hasConceptScore W2064570845C43617362 @default.
- W2064570845 hasConceptScore W2064570845C55493867 @default.
- W2064570845 hasConceptScore W2064570845C58121356 @default.