FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064592675> ?p ?o ?g. }

• W2064592675 endingPage "1055" @default.
• W2064592675 startingPage "1045" @default.
• W2064592675 abstract "Self-cleavage of the genomic and antigenomic ribozymes from hepatitis delta virus (HDV) requires divalent cation for optimal activity. Recently, the HDV genomic ribozyme has been shown to be active in NaCl in the absence of added divalent metal ion at low pH (apparent pKa 5.7). However, we find that the antigenomic ribozyme is 100 to 1000-fold less active under similar conditions. With deletion of a three-nucleotide sequence (C41-A42-A43) unique to the genomic ribozyme, the rate constant for cleavage decreased substantially, while activity of the antigenomic ribozyme was enhanced by introducing a CAA sequence. From the crystal structure, it has been proposed that C41 in this sequence is protonated. To investigate a possible connection between activity at low pH and protonation of C41, mutations were made that were predicted to either eliminate protonation or alter the nature of the tertiary interaction upon protonation. In the absence of added Mg2+, these mutations reduced activity and eliminated the observed pH-rate dependence. Thermal denaturation studies revealed a pH-sensitive structural feature in the genomic ribozyme, while unfolding of the mutant ribozymes was pH-independent. We propose that, in the absence of added Mg2+, protonation of C41 contributes to enhanced activity of the HDV genomic ribozyme at low pH." @default.
• W2064592675 created "2016-06-24" @default.
• W2064592675 creator A5004944798 @default.
• W2064592675 creator A5026027351 @default.
• W2064592675 creator A5081088894 @default.
• W2064592675 creator A5088506167 @default.
• W2064592675 date "2001-02-01" @default.
• W2064592675 modified "2023-09-26" @default.
• W2064592675 title "A pH-sensitive RNA tertiary interaction affects self-cleavage activity of the HDV ribozymes in the absence of added divalent metal ion" @default.
• W2064592675 cites W1487939329 @default.
• W2064592675 cites W1502906266 @default.
• W2064592675 cites W1555021163 @default.
• W2064592675 cites W1569125732 @default.
• W2064592675 cites W1678644792 @default.
• W2064592675 cites W1881326155 @default.
• W2064592675 cites W1974000520 @default.
• W2064592675 cites W1975389196 @default.
• W2064592675 cites W1984005624 @default.
• W2064592675 cites W1995675684 @default.
• W2064592675 cites W2030432262 @default.
• W2064592675 cites W2043951259 @default.
• W2064592675 cites W2053471524 @default.
• W2064592675 cites W2054402826 @default.
• W2064592675 cites W2068797168 @default.
• W2064592675 cites W2078224461 @default.
• W2064592675 cites W2092876446 @default.
• W2064592675 cites W2104214639 @default.
• W2064592675 cites W2104746849 @default.
• W2064592675 cites W2120883316 @default.
• W2064592675 cites W2121697003 @default.
• W2064592675 cites W2124512134 @default.
• W2064592675 cites W2888511473 @default.
• W2064592675 cites W4212901177 @default.
• W2064592675 doi "https://doi.org/10.1006/jmbi.2000.4368" @default.
• W2064592675 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11162113" @default.
• W2064592675 hasPublicationYear "2001" @default.
• W2064592675 type Work @default.
• W2064592675 sameAs 2064592675 @default.
• W2064592675 citedByCount "79" @default.
• W2064592675 countsByYear W20645926752012 @default.
• W2064592675 countsByYear W20645926752013 @default.
• W2064592675 countsByYear W20645926752015 @default.
• W2064592675 countsByYear W20645926752020 @default.
• W2064592675 crossrefType "journal-article" @default.
• W2064592675 hasAuthorship W2064592675A5004944798 @default.
• W2064592675 hasAuthorship W2064592675A5026027351 @default.
• W2064592675 hasAuthorship W2064592675A5081088894 @default.
• W2064592675 hasAuthorship W2064592675A5088506167 @default.
• W2064592675 hasConcept C104317684 @default.
• W2064592675 hasConcept C105130999 @default.
• W2064592675 hasConcept C145148216 @default.
• W2064592675 hasConcept C151730666 @default.
• W2064592675 hasConcept C175156509 @default.
• W2064592675 hasConcept C178790620 @default.
• W2064592675 hasConcept C185592680 @default.
• W2064592675 hasConcept C30095370 @default.
• W2064592675 hasConcept C30951146 @default.
• W2064592675 hasConcept C43369102 @default.
• W2064592675 hasConcept C512185932 @default.
• W2064592675 hasConcept C55493867 @default.
• W2064592675 hasConcept C66144319 @default.
• W2064592675 hasConcept C67705224 @default.
• W2064592675 hasConcept C71240020 @default.
• W2064592675 hasConcept C74884574 @default.
• W2064592675 hasConcept C76346623 @default.
• W2064592675 hasConcept C8010536 @default.
• W2064592675 hasConcept C86803240 @default.
• W2064592675 hasConceptScore W2064592675C104317684 @default.
• W2064592675 hasConceptScore W2064592675C105130999 @default.
• W2064592675 hasConceptScore W2064592675C145148216 @default.
• W2064592675 hasConceptScore W2064592675C151730666 @default.
• W2064592675 hasConceptScore W2064592675C175156509 @default.
• W2064592675 hasConceptScore W2064592675C178790620 @default.
• W2064592675 hasConceptScore W2064592675C185592680 @default.
• W2064592675 hasConceptScore W2064592675C30095370 @default.
• W2064592675 hasConceptScore W2064592675C30951146 @default.
• W2064592675 hasConceptScore W2064592675C43369102 @default.
• W2064592675 hasConceptScore W2064592675C512185932 @default.
• W2064592675 hasConceptScore W2064592675C55493867 @default.
• W2064592675 hasConceptScore W2064592675C66144319 @default.
• W2064592675 hasConceptScore W2064592675C67705224 @default.
• W2064592675 hasConceptScore W2064592675C71240020 @default.
• W2064592675 hasConceptScore W2064592675C74884574 @default.
• W2064592675 hasConceptScore W2064592675C76346623 @default.
• W2064592675 hasConceptScore W2064592675C8010536 @default.
• W2064592675 hasConceptScore W2064592675C86803240 @default.
• W2064592675 hasIssue "5" @default.
• W2064592675 hasLocation W20645926751 @default.
• W2064592675 hasLocation W20645926752 @default.
• W2064592675 hasOpenAccess W2064592675 @default.
• W2064592675 hasPrimaryLocation W20645926751 @default.
• W2064592675 hasRelatedWork W2007638725 @default.
• W2064592675 hasRelatedWork W2025905252 @default.
• W2064592675 hasRelatedWork W2045000926 @default.
• W2064592675 hasRelatedWork W2052970102 @default.
• W2064592675 hasRelatedWork W2053576304 @default.
• W2064592675 hasRelatedWork W2064592675 @default.
• W2064592675 hasRelatedWork W2087572417 @default.

• next