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• W2064731567 abstract "The Salmonella PmrA/PmrB two-component system uses an iron(III)-binding motif on the cell surface to sense the environmental or host ferric level and regulate PmrA-controlled gene expression. We replaced the iron(III)-binding motif with a lanthanide-binding peptide sequence that is known to selectively recognize trivalent lanthanide ions. The newly engineered two-component system (PmrA/PmrB) can effectively sense lanthanide ion and regulate gene expression in E. coli . This work not only provides the first known lanthanide-based sensing and response in live cells but also demonstrates that the PmrA/PmrB system is a suitable template for future synthetic biology efforts to construct bacteria that can sense and respond to other metal ions in remediation or sequestration." @default.
• W2064731567 created "2016-06-24" @default.
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• W2064731567 date "2013-02-01" @default.
• W2064731567 modified "2023-10-18" @default.
• W2064731567 title "Engineering Bacterial Two-Component System PmrA/PmrB to Sense Lanthanide Ions" @default.
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• W2064731567 doi "https://doi.org/10.1021/ja312032c" @default.
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