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- W2064751784 abstract "A new method for estimating collagen synthesis in microwell cultures of fibroblasts is presented, 3H-Labeled-proline-collagen was purified by successive salt precipitations at acid and neutral pH in the presence of carrier collagen. Variability between replicates was less than 10% (standard deviation) and recovery of labeled collage internal standards was greater than 90%. More than 90% of recoverable radioactivity was in collagen as demonstrated by polyacrylamide gel electrophoresis and carboxymethyl cellulose chromatography. The culture system is highly reproducible and allows use of a large number of separate cultures with uniformity of culture conditions and economy of reagents." @default.
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- W2064751784 title "A quantitative assay for collagen synthesis in microwell fibroblast cultures" @default.
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- W2064751784 doi "https://doi.org/10.1016/0003-2697(79)90576-1" @default.
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