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• W2064762260 abstract "Background: The function of a reconstructed bladder depends on the contracting bladder wall. This can be obtained with sufficient angiogenesis. Polyglycolic acid (PGA) is used as a cell vehicle for bladder reconstruction. Chitosan supports adhesion and differentiation of endothelial cells. The aim of the study was to compare PGA with PGA/chitosan ‘sandwich’ grafts for bladder wall regeneration. Materials and Methods: 3T3 fibroblasts were seeded on 6 PGA and 3 chitosan scaffolds and incubated 3 days at 37°C and 5% CO2 before implantation. Three rats underwent bladder reconstruction with PGA cell seeded grafts and 3 with PGA grafts covered with chitosan cell seeded grafts (‘sandwich’ graft). Three rats as controls were not operated on. After 6 months reconstructed tissue was stained with H&E. Vessels were identified by immunohistochemistry by CD31 and CD34 staining. A 4-level scale was used, (+++) referred to evident marker presence (high density of vessels) and (-) as lack of a marker (no vessels). Results: No complications during the 6- month period were noted. PGA/chitosan grafts were evaluated as (+) while PGA grafts were evaluated as (+) and (-) by the use of the CD31 antibody. The control group was evaluated as (+). PGA/chitosan grafts were evaluated as (++) and (+) while PGA grafts were evaluated as (++) and (-) by CD34 staining. The control group was evaluated as (+) and (-). Conclusion: Chitosan improved PGA abilities as a cell matrix and enhanced angiogenesis within the in vitro reconstructed graft." @default.
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• W2064762260 date "2008-01-01" @default.
• W2064762260 modified "2023-10-18" @default.
• W2064762260 title "Chitosan Scaffold Enhances Angiogenesis within an in vitro Reconstructed Bladder Wall, an Animal Study" @default.
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• W2064762260 doi "https://doi.org/10.1159/000106537" @default.
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