FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064791021> ?p ?o ?g. }

• W2064791021 endingPage "4769" @default.
• W2064791021 startingPage "4764" @default.
• W2064791021 abstract "This investigation was pursued to test the use of intracellular antibodies (intrabodies) as a means of blocking the pathogenesis of Huntington's disease (HD). HD is characterized by abnormally elongated polyglutamine near the N terminus of the huntingtin protein, which induces pathological protein-protein interactions and aggregate formation by huntingtin or its exon 1-containing fragments. Selection from a large human phage display library yielded a single-chain Fv (sFv) antibody specific for the 17 N-terminal residues of huntingtin, adjacent to the polyglutamine in HD exon 1. This anti-huntingtin sFv intrabody was tested in a cellular model of the disease in which huntingtin exon 1 had been fused to green fluorescent protein (GFP). Expression of expanded repeat HD-polyQ-GFP in transfected cells shows perinuclear aggregation similar to human HD pathology, which worsens with increasing polyglutamine length; the number of aggregates in these transfected cells provided a quantifiable model of HD for this study. Coexpression of anti-huntingtin sFv intrabodies with the abnormal huntingtin-GFP fusion protein dramatically reduced the number of aggregates, compared with controls lacking the intrabody. Anti-huntingtin sFv fused with a nuclear localization signal retargeted huntingtin analogues to cell nuclei, providing further evidence of the anti-huntingtin sFv specificity and of its capacity to redirect the subcellular localization of exon 1. This study suggests that intrabody-mediated modulation of abnormal neuronal proteins may contribute to the treatment of neurodegenerative diseases such as HD, Alzheimer's, Parkinson's, prion disease, and the spinocerebellar ataxias." @default.
• W2064791021 created "2016-06-24" @default.
• W2064791021 creator A5003912060 @default.
• W2064791021 creator A5016442374 @default.
• W2064791021 creator A5019220405 @default.
• W2064791021 creator A5033759527 @default.
• W2064791021 creator A5054562347 @default.
• W2064791021 creator A5055838559 @default.
• W2064791021 creator A5063025236 @default.
• W2064791021 creator A5064625635 @default.
• W2064791021 date "2001-04-10" @default.
• W2064791021 modified "2023-10-13" @default.
• W2064791021 title "Human single-chain Fv intrabodies counteract <i>in situ</i> huntingtin aggregation in cellular models of Huntington's disease" @default.
• W2064791021 cites W17705042 @default.
• W2064791021 cites W1820767944 @default.
• W2064791021 cites W1940885739 @default.
• W2064791021 cites W1962249623 @default.
• W2064791021 cites W1985474993 @default.
• W2064791021 cites W1989669244 @default.
• W2064791021 cites W1992526113 @default.
• W2064791021 cites W1996789718 @default.
• W2064791021 cites W1999745373 @default.
• W2064791021 cites W2001299005 @default.
• W2064791021 cites W2002644780 @default.
• W2064791021 cites W2005927762 @default.
• W2064791021 cites W2013285346 @default.
• W2064791021 cites W2030410903 @default.
• W2064791021 cites W2037562033 @default.
• W2064791021 cites W2041993377 @default.
• W2064791021 cites W2042716629 @default.
• W2064791021 cites W2044356662 @default.
• W2064791021 cites W2056565619 @default.
• W2064791021 cites W2062554378 @default.
• W2064791021 cites W2062682951 @default.
• W2064791021 cites W2080431132 @default.
• W2064791021 cites W2082501455 @default.
• W2064791021 cites W2089204594 @default.
• W2064791021 cites W2099997349 @default.
• W2064791021 cites W2121642311 @default.
• W2064791021 cites W2123631149 @default.
• W2064791021 cites W2135718381 @default.
• W2064791021 cites W2154216619 @default.
• W2064791021 cites W2156079900 @default.
• W2064791021 cites W2170726804 @default.
• W2064791021 cites W2171370174 @default.
• W2064791021 cites W2171466517 @default.
• W2064791021 cites W3003718293 @default.
• W2064791021 doi "https://doi.org/10.1073/pnas.071058398" @default.
• W2064791021 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/31908" @default.
• W2064791021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11296304" @default.
• W2064791021 hasPublicationYear "2001" @default.
• W2064791021 type Work @default.
• W2064791021 sameAs 2064791021 @default.
• W2064791021 citedByCount "180" @default.
• W2064791021 countsByYear W20647910212012 @default.
• W2064791021 countsByYear W20647910212013 @default.
• W2064791021 countsByYear W20647910212014 @default.
• W2064791021 countsByYear W20647910212015 @default.
• W2064791021 countsByYear W20647910212016 @default.
• W2064791021 countsByYear W20647910212017 @default.
• W2064791021 countsByYear W20647910212018 @default.
• W2064791021 countsByYear W20647910212019 @default.
• W2064791021 countsByYear W20647910212020 @default.
• W2064791021 countsByYear W20647910212021 @default.
• W2064791021 countsByYear W20647910212022 @default.
• W2064791021 countsByYear W20647910212023 @default.
• W2064791021 crossrefType "journal-article" @default.
• W2064791021 hasAuthorship W2064791021A5003912060 @default.
• W2064791021 hasAuthorship W2064791021A5016442374 @default.
• W2064791021 hasAuthorship W2064791021A5019220405 @default.
• W2064791021 hasAuthorship W2064791021A5033759527 @default.
• W2064791021 hasAuthorship W2064791021A5054562347 @default.
• W2064791021 hasAuthorship W2064791021A5055838559 @default.
• W2064791021 hasAuthorship W2064791021A5063025236 @default.
• W2064791021 hasAuthorship W2064791021A5064625635 @default.
• W2064791021 hasBestOaLocation W20647910211 @default.
• W2064791021 hasConcept C104317684 @default.
• W2064791021 hasConcept C123894998 @default.
• W2064791021 hasConcept C142613039 @default.
• W2064791021 hasConcept C142724271 @default.
• W2064791021 hasConcept C143065580 @default.
• W2064791021 hasConcept C153911025 @default.
• W2064791021 hasConcept C2779134260 @default.
• W2064791021 hasConcept C2780647506 @default.
• W2064791021 hasConcept C2781427258 @default.
• W2064791021 hasConcept C2910011555 @default.
• W2064791021 hasConcept C36823959 @default.
• W2064791021 hasConcept C40767141 @default.
• W2064791021 hasConcept C54009773 @default.
• W2064791021 hasConcept C54355233 @default.
• W2064791021 hasConcept C71924100 @default.
• W2064791021 hasConcept C86803240 @default.
• W2064791021 hasConcept C95444343 @default.
• W2064791021 hasConceptScore W2064791021C104317684 @default.
• W2064791021 hasConceptScore W2064791021C123894998 @default.
• W2064791021 hasConceptScore W2064791021C142613039 @default.
• W2064791021 hasConceptScore W2064791021C142724271 @default.
• W2064791021 hasConceptScore W2064791021C143065580 @default.

• next