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- W2064804926 abstract "A cell-free pre-mRNA splicing system was developed from the nuclear extract of Bombyx mori fat bodies. The synthetic pre-mRNA from the cytoplasmic actin gene of B. mori (BmA3 ) was used as a substrate for in vitro splicing. The pre-mRNA was accurately spliced in the nuclear extract prepared from the fat body. Sequencing of the amplicon by RT-PCR revealed that the spliced site in vitro was identical to the site in vivo. We found that a part of the input capped pre-mRNA was accurately spliced after 2h of incubation. In addition, the RT-PCR analysis precisely mapped the branch point to an adenine located at 30 nucleotides before the 3' end of the first intron in BmA3. This system was a first example that differentiated tissue in insects yielded active extracts for in vitro splicing." @default.
- W2064804926 created "2016-06-24" @default.
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- W2064804926 date "2005-03-01" @default.
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- W2064804926 title "Accurate pre-mRNA splicing using a nuclear extract from Bombyx mori fat body" @default.
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- W2064804926 doi "https://doi.org/10.1016/j.ibmb.2004.12.005" @default.
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