Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064960882> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2064960882 endingPage "49a" @default.
- W2064960882 startingPage "48a" @default.
- W2064960882 abstract "Lipoprotein Signal Peptidase A (LspA) is a type II signal peptidase that is an essential enzyme in one of the three distinct steps in lipoprotein maturation. After targeting the prolipoprotein, LspA cleaves the signal peptide of lipoprotein to produce the precursor for the mature lipoprotein. In Mycobacterium tuberculosis (Mtb), gene Rv1539 encodes LspA, an inner membrane protein of 21 kDa (202 amino acid residues). In vivo and in vitro experimental evidence confirms that LspA is essential for Mtb and its contribution for the antibiotic resistance. Small molecules have access to the active site of this enzyme which is located extracellular to the cytoplasmic membrane. All type II signal peptidases have five conserved domains but the absence of those domains in eukaryotic cells makes LspA an appealing drug target for the treatment of tuberculosis. Globomycin, a cyclic-peptide can inhibit LspA in a non-competitive manner. The structure of small helical membrane proteins is very sensitive to their environment and therefore it is vital to characterize the structure them in a native-like environment, such as a synthetic lipid bilayer or a bacterial cell membrane. Solid state NMR spectroscopy is able to make such structural characterizations in such environments. Here, the challenging, sample preparation steps are described and initial Magic Angle Spinning solid state NMR spectra are reported from 13C isotopically labeled LspA in native E.coli and synthetic lipid bilayers. In addition, uniformly oriented samples for solid state NMR spectra of 15N isotopically labeled LspA, have been prepared in synthetic lipid bilayers providing initial structural insights." @default.
- W2064960882 created "2016-06-24" @default.
- W2064960882 creator A5004910720 @default.
- W2064960882 creator A5020704363 @default.
- W2064960882 creator A5072491046 @default.
- W2064960882 creator A5073090269 @default.
- W2064960882 date "2014-01-01" @default.
- W2064960882 modified "2023-09-30" @default.
- W2064960882 title "Structural Insights of LspA, from Mycobacterium Tuberculosis, using Solid State NMR Spectroscopy" @default.
- W2064960882 doi "https://doi.org/10.1016/j.bpj.2013.11.348" @default.
- W2064960882 hasPublicationYear "2014" @default.
- W2064960882 type Work @default.
- W2064960882 sameAs 2064960882 @default.
- W2064960882 citedByCount "0" @default.
- W2064960882 crossrefType "journal-article" @default.
- W2064960882 hasAuthorship W2064960882A5004910720 @default.
- W2064960882 hasAuthorship W2064960882A5020704363 @default.
- W2064960882 hasAuthorship W2064960882A5072491046 @default.
- W2064960882 hasAuthorship W2064960882A5073090269 @default.
- W2064960882 hasBestOaLocation W20649608821 @default.
- W2064960882 hasConcept C104317684 @default.
- W2064960882 hasConcept C10858879 @default.
- W2064960882 hasConcept C121332964 @default.
- W2064960882 hasConcept C146587185 @default.
- W2064960882 hasConcept C153202636 @default.
- W2064960882 hasConcept C161624437 @default.
- W2064960882 hasConcept C167625842 @default.
- W2064960882 hasConcept C185592680 @default.
- W2064960882 hasConcept C2779190341 @default.
- W2064960882 hasConcept C2779281246 @default.
- W2064960882 hasConcept C39944091 @default.
- W2064960882 hasConcept C41625074 @default.
- W2064960882 hasConcept C46141821 @default.
- W2064960882 hasConcept C547475151 @default.
- W2064960882 hasConcept C55493867 @default.
- W2064960882 hasConcept C66974803 @default.
- W2064960882 hasConcept C71240020 @default.
- W2064960882 hasConcept C86803240 @default.
- W2064960882 hasConceptScore W2064960882C104317684 @default.
- W2064960882 hasConceptScore W2064960882C10858879 @default.
- W2064960882 hasConceptScore W2064960882C121332964 @default.
- W2064960882 hasConceptScore W2064960882C146587185 @default.
- W2064960882 hasConceptScore W2064960882C153202636 @default.
- W2064960882 hasConceptScore W2064960882C161624437 @default.
- W2064960882 hasConceptScore W2064960882C167625842 @default.
- W2064960882 hasConceptScore W2064960882C185592680 @default.
- W2064960882 hasConceptScore W2064960882C2779190341 @default.
- W2064960882 hasConceptScore W2064960882C2779281246 @default.
- W2064960882 hasConceptScore W2064960882C39944091 @default.
- W2064960882 hasConceptScore W2064960882C41625074 @default.
- W2064960882 hasConceptScore W2064960882C46141821 @default.
- W2064960882 hasConceptScore W2064960882C547475151 @default.
- W2064960882 hasConceptScore W2064960882C55493867 @default.
- W2064960882 hasConceptScore W2064960882C66974803 @default.
- W2064960882 hasConceptScore W2064960882C71240020 @default.
- W2064960882 hasConceptScore W2064960882C86803240 @default.
- W2064960882 hasIssue "2" @default.
- W2064960882 hasLocation W20649608821 @default.
- W2064960882 hasOpenAccess W2064960882 @default.
- W2064960882 hasPrimaryLocation W20649608821 @default.
- W2064960882 hasRelatedWork W1497574444 @default.
- W2064960882 hasRelatedWork W1849062936 @default.
- W2064960882 hasRelatedWork W2021501853 @default.
- W2064960882 hasRelatedWork W2023807453 @default.
- W2064960882 hasRelatedWork W2038444889 @default.
- W2064960882 hasRelatedWork W2041543210 @default.
- W2064960882 hasRelatedWork W2064960882 @default.
- W2064960882 hasRelatedWork W2072604958 @default.
- W2064960882 hasRelatedWork W2092962465 @default.
- W2064960882 hasRelatedWork W2572680590 @default.
- W2064960882 hasVolume "106" @default.
- W2064960882 isParatext "false" @default.
- W2064960882 isRetracted "false" @default.
- W2064960882 magId "2064960882" @default.
- W2064960882 workType "article" @default.