FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2064996852> ?p ?o ?g. }

• W2064996852 endingPage "e57851" @default.
• W2064996852 startingPage "e57851" @default.
• W2064996852 abstract "According to traditional murine bioassay methodology, prions must be serially passaged within a new host before a stable phenotype, and therefore a strain, can be assigned. Prions often transmit with difficulty from one species to another; a property termed the transmission barrier. Transgenic mouse lines that over express prion protein (PrP) genes of different species can circumvent the transmission barrier but serial passages may still be required, particularly if unknown strains are encountered. Here we sought to investigate whether protein misfolding cyclic amplification (PMCA), an in-vitro method of PrPSc replication, could be used to replace serial passage of VRQ/VRQ classical scrapie isolates undergoing strain typing in ovine transgenic tg338 mice. Two classical scrapie field isolates that do not readily transmit to wild-type mice underwent bioassay in tg338 mice pre- and post- PMCA and the phenotype of disease in inoculated mice was compared. For one of the sources investigated, the PMCA product gave rise to the same disease phenotypes in tg338 mice as traditional bioassay, as indicated by lesion profile, IHC analysis and Western blot, whilst the second source produced phenotypic characteristics which were not identical with those that arose through traditional bioassay. These data show that differences in the efficiency of PMCA as a strain-typing tool may vary between ovine classical scrapie isolates and therefore suggest that the ability of PMCA to replace serial passage of classical scrapie in tg338 mice may depend on the strain present in the initial source." @default.
• W2064996852 created "2016-06-24" @default.
• W2064996852 creator A5001063055 @default.
• W2064996852 creator A5035653594 @default.
• W2064996852 creator A5036556436 @default.
• W2064996852 creator A5070725092 @default.
• W2064996852 creator A5071285751 @default.
• W2064996852 creator A5075911501 @default.
• W2064996852 creator A5080427948 @default.
• W2064996852 date "2013-03-05" @default.
• W2064996852 modified "2023-10-18" @default.
• W2064996852 title "Strain Typing of Classical Scrapie by Transgenic Mouse Bioassay Using Protein Misfolding Cyclic Amplification to Replace Primary Passage" @default.
• W2064996852 cites W1624018543 @default.
• W2064996852 cites W1965257173 @default.
• W2064996852 cites W1967254771 @default.
• W2064996852 cites W1968142614 @default.
• W2064996852 cites W1989150559 @default.
• W2064996852 cites W1991644458 @default.
• W2064996852 cites W2003537472 @default.
• W2064996852 cites W2003884554 @default.
• W2064996852 cites W2022392367 @default.
• W2064996852 cites W2027555709 @default.
• W2064996852 cites W2036645353 @default.
• W2064996852 cites W2048460135 @default.
• W2064996852 cites W2049719457 @default.
• W2064996852 cites W2052293684 @default.
• W2064996852 cites W2054556707 @default.
• W2064996852 cites W2058503712 @default.
• W2064996852 cites W2064366827 @default.
• W2064996852 cites W2067540111 @default.
• W2064996852 cites W2078164511 @default.
• W2064996852 cites W2081825344 @default.
• W2064996852 cites W2084388744 @default.
• W2064996852 cites W2095358223 @default.
• W2064996852 cites W2109938262 @default.
• W2064996852 cites W2110516310 @default.
• W2064996852 cites W2130432938 @default.
• W2064996852 cites W2138468217 @default.
• W2064996852 cites W2154521232 @default.
• W2064996852 cites W2156464988 @default.
• W2064996852 cites W2162615082 @default.
• W2064996852 cites W2169849112 @default.
• W2064996852 cites W2303163021 @default.
• W2064996852 doi "https://doi.org/10.1371/journal.pone.0057851" @default.
• W2064996852 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3589467" @default.
• W2064996852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23472112" @default.
• W2064996852 hasPublicationYear "2013" @default.
• W2064996852 type Work @default.
• W2064996852 sameAs 2064996852 @default.
• W2064996852 citedByCount "10" @default.
• W2064996852 countsByYear W20649968522014 @default.
• W2064996852 countsByYear W20649968522015 @default.
• W2064996852 countsByYear W20649968522016 @default.
• W2064996852 countsByYear W20649968522018 @default.
• W2064996852 countsByYear W20649968522019 @default.
• W2064996852 countsByYear W20649968522021 @default.
• W2064996852 countsByYear W20649968522022 @default.
• W2064996852 crossrefType "journal-article" @default.
• W2064996852 hasAuthorship W2064996852A5001063055 @default.
• W2064996852 hasAuthorship W2064996852A5035653594 @default.
• W2064996852 hasAuthorship W2064996852A5036556436 @default.
• W2064996852 hasAuthorship W2064996852A5070725092 @default.
• W2064996852 hasAuthorship W2064996852A5071285751 @default.
• W2064996852 hasAuthorship W2064996852A5075911501 @default.
• W2064996852 hasAuthorship W2064996852A5080427948 @default.
• W2064996852 hasBestOaLocation W20649968521 @default.
• W2064996852 hasConcept C102230213 @default.
• W2064996852 hasConcept C104317684 @default.
• W2064996852 hasConcept C104488531 @default.
• W2064996852 hasConcept C105702510 @default.
• W2064996852 hasConcept C127716648 @default.
• W2064996852 hasConcept C141035611 @default.
• W2064996852 hasConcept C142724271 @default.
• W2064996852 hasConcept C153911025 @default.
• W2064996852 hasConcept C159047783 @default.
• W2064996852 hasConcept C2776735649 @default.
• W2064996852 hasConcept C2778022156 @default.
• W2064996852 hasConcept C2779134260 @default.
• W2064996852 hasConcept C3019804061 @default.
• W2064996852 hasConcept C54355233 @default.
• W2064996852 hasConcept C71924100 @default.
• W2064996852 hasConcept C86803240 @default.
• W2064996852 hasConceptScore W2064996852C102230213 @default.
• W2064996852 hasConceptScore W2064996852C104317684 @default.
• W2064996852 hasConceptScore W2064996852C104488531 @default.
• W2064996852 hasConceptScore W2064996852C105702510 @default.
• W2064996852 hasConceptScore W2064996852C127716648 @default.
• W2064996852 hasConceptScore W2064996852C141035611 @default.
• W2064996852 hasConceptScore W2064996852C142724271 @default.
• W2064996852 hasConceptScore W2064996852C153911025 @default.
• W2064996852 hasConceptScore W2064996852C159047783 @default.
• W2064996852 hasConceptScore W2064996852C2776735649 @default.
• W2064996852 hasConceptScore W2064996852C2778022156 @default.
• W2064996852 hasConceptScore W2064996852C2779134260 @default.
• W2064996852 hasConceptScore W2064996852C3019804061 @default.
• W2064996852 hasConceptScore W2064996852C54355233 @default.
• W2064996852 hasConceptScore W2064996852C71924100 @default.
• W2064996852 hasConceptScore W2064996852C86803240 @default.

• next