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- W2065012124 abstract "Comparative global proteome analyses were performed on Leptospira interrogans serovar Copenhageni grown under conventional in vitro conditions and those mimicking in vivo conditions (iron limitation and serum presence). Proteomic analyses were conducted using iTRAQ and LC-ESI-tandem mass spectrometry complemented with two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. A total of 563 proteins were identified in this study. Altered expression of 65 proteins, including upregulation of the L. interrogans virulence factor Loa22 and 5 novel proteins with homology to virulence factors found in other pathogens, was observed between the comparative conditions. Immunoblot analyses confirmed upregulation of 5 of the known or putative virulence factors in L. interrogans exposed to the in vivo-like environmental conditions. Further, ELISA analyses using serum from patients with leptospirosis and immunofluorescence studies performed on liver sections derived from L. interrogans-infected hamsters verified expression of all but one of the identified proteins during infection. These studies, which represent the first documented comparative global proteome analysis of Leptospira, demonstrated proteome alterations under conditions that mimic in vivo infection and allowed for the identification of novel putative L. interrogans virulence factors." @default.
- W2065012124 created "2016-06-24" @default.
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- W2065012124 date "2009-08-24" @default.
- W2065012124 modified "2023-10-16" @default.
- W2065012124 title "Global Proteome Analysis of <i>Leptospira interrogans</i>" @default.
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- W2065012124 doi "https://doi.org/10.1021/pr9004597" @default.
- W2065012124 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2757032" @default.
- W2065012124 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19663501" @default.
- W2065012124 hasPublicationYear "2009" @default.
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