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• W2065016143 abstract "Novel silica-coated luminescent europium (SCLE) nanoparticles have been prepared by copolymerization of tetraethyl orthosilicate and 3-aminopropyl(triethoxy)silane with ammonium hydroxide in a water-in-oil microemulsion containing a luminescent Eu3+ chelate, Triton X-100, n-octanol, and cyclohexane. The nanoparticles were characterized by transmission electron microscopy, X-ray diffraction, and UV−vis and luminescence spectra. The results show that the nanoparticles are spherical and uniform in size, 50 ± 5 nm in diameter, and have high photostability and long luminescence lifetime (770 μs). Because primary amino groups have been directly introduced to the nanoparticle's surface by using 3-aminopropyl(triethoxyl)silane in nanoparticle preparation, the surface modification and bioconjugation of the nanoparticles are easier and reproducible. The nanoparticles were used for streptavidin (SA) labeling, and the nanoparticle-labeled streptavidin was successfully used in time-resolved fluoroimmunoassay of human α-fetoprotein. The results reveal that the new nanoparticles are favorable for use as a new type of luminescence probe for highly sensitive time-resolved luminescence bioassay." @default.
• W2065016143 created "2016-06-24" @default.
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• W2065016143 date "2004-05-18" @default.
• W2065016143 modified "2023-10-14" @default.
• W2065016143 title "Preparation and Time-Resolved Fluorometric Application of Luminescent Europium Nanoparticles" @default.
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• W2065016143 doi "https://doi.org/10.1021/cm030305n" @default.
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