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- W2065033403 abstract "Indoleacetic acid (IAA) oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) grown on a medium supplemented with gibberellic acid (GA 3 ), IAA, and kinetin was resolved into seven isoenzymes by polyacrylamide gel electrophoresis. Gibberellic acid promoted the development of three fast-migrating IAA oxidase isoenzymes which were 7- to 10-fold more active than those of the control. Gibberellic acid was effective at 2 × 10 −9 M and its activity increased with increasing concentration up to 2 × 10 −7 M. Actinomycin D and cycloheximide inhibited the development of these isoenzymes, suggesting a requirement for both RNA and protein synthesis. The GA 3 -promoted IAA oxidase isoenzymes increased with time and were positively associated with the GA 3 -induced characteristic type of growth.The expression of the GA 3 effect on IAA oxidase isoenzymes and growth was dependent on IAA and kinetin. In the presence of appropriate concentrations of kinetin, the combined effect of GA 3 and IAA was more than additive. A possible interpretation of the GA 3 –IAA interaction is discussed." @default.
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- W2065033403 date "1971-05-01" @default.
- W2065033403 modified "2023-09-27" @default.
- W2065033403 title "Increase of indoleacetic acid oxidase isoenzymes by gibberellic acid in tobacco callus cultures" @default.
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- W2065033403 doi "https://doi.org/10.1139/b71-106" @default.
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