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- W2065073949 abstract "During transcription initiation, the promoter DNA is recognized and bent by the basal transcription factor TATA-binding protein (TBP). Subsequent association of transcription factor B (TFB) with the TBP–DNA complex is followed by the recruitment of the ribonucleic acid polymerase resulting in the formation of the pre-initiation complex. TBP and TFB/TF(II)B are highly conserved in structure and function among the eukaryotic-archaeal domain but intriguingly have to operate under vastly different conditions. Employing single-pair fluorescence resonance energy transfer, we monitored DNA bending by eukaryotic and archaeal TBPs in the absence and presence of TFB in real-time. We observed that the lifetime of the TBP–DNA interaction differs significantly between the archaeal and eukaryotic system. We show that the eukaryotic DNA-TBP interaction is characterized by a linear, stepwise bending mechanism with an intermediate state distinguished by a distinct bending angle. TF(II)B specifically stabilizes the fully bent TBP–promoter DNA complex and we identify this step as a regulatory checkpoint. In contrast, the archaeal TBP–DNA interaction is extremely dynamic and TBP from the archaeal organism Sulfolobus acidocaldarius strictly requires TFB for DNA bending. Thus, we demonstrate that transcription initiation follows diverse pathways on the way to the formation of the pre-initiation complex." @default.
- W2065073949 created "2016-06-24" @default.
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- W2065073949 date "2014-04-17" @default.
- W2065073949 modified "2023-10-16" @default.
- W2065073949 title "Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways" @default.
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- W2065073949 doi "https://doi.org/10.1093/nar/gku273" @default.
- W2065073949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4041446" @default.
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