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- W2065089080 abstract "One of the key pathogenicity factors of the cholera pathogen is the cholera toxin (CT), which is encoded by the operon ctxAB contained in the CTXφ prophage. Variations in the CTXφ prophage genome, the core region of which contains ctxAB, zot, ace, cep, orfU, and psh genes, underlies the changes in virulent properties of cholera vibrios. The mechanisms involved in remodeling this prophage genome are still vague. In this work, we demonstrate that the insertion of Tn5-Mob (KmR) transposon into the chromosome of the Vibrio cholerae model strain MAK757 biovar El Tor, which carries two copies of the CTXφ prophage, induces the remodeling of the prophage genome, namely, the deletion of the zot, ace, cep, and orfU genes. The level of CT biosynthesis in the insertion mutants MAK757 chr::Tn5-Mob, which only retain the ctxAB operon, increased more than 2000-fold as compared to the original strain. It has been shown that the increased CT production is associated with the altered structure of the chromosomal DNA region containing a copy of the ctxAB operon, encoding this protein. This mutation in the genome of CTXφ prophage induced by Tn5-Mob is unstable. Among the 600 isolated colonies obtained after screening the MAK757 chr::Tn5-Mob transposant capable of CT overproduction on a full medium free of antibiotics, 5.8% yielded clones that lost the ctxAB operon, as well as the marker KmR, thus becoming nontoxigenic. The emergence of V. cholerae mutants both that both overproduce CT and are nontoxigenic demonstrates the important role of genetic variations in the CTXφ prophage in the evolution of V. cholerae pathogenicity. The obtained plasmid-free strain of V. cholerae biovar El Tor with type-2 CToverproduction can be used as a producer of this toxin, which is used to manufacture preparations for cholera diagnostics and prevention." @default.
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- W2065089080 date "2008-09-01" @default.
- W2065089080 modified "2023-10-16" @default.
- W2065089080 title "Genomic variations of CTXφ prophage of Vibrio cholerae biovar El tor induced by Tn5-Mob transposone" @default.
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- W2065089080 doi "https://doi.org/10.3103/s0891416808030026" @default.
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