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• W2065111539 abstract "We prepared a β-1,3-glucan oligomer (DP/>-4) from laminarin (DP: 25–30) derived from Laminaria digitata with β-1,3-glucanase, and examined its effect on human peripheral blood monocytes. Conditioned medium prepared by incubating monocytes (MC-CM) with the β-1,3-glucan oligomer showed strong inhibitory activity against the proliferation of human leukemic U937 cells. Since the β-1,3-glucan oligomer had no direct cytotoxic effect on U937 cells up to 1000 μg/ml, the cytotoxicity of the MC-CM may be due to cytotoxic cytokines produced from monocytes stimulated by the β-1,3-glucan oligomer. On the other hand, the MC-CM prepared with original laminarin had little effect on the growth of U937 cells. The cytotoxicity of the MC-CM prepared with the β-1,3-glucan oligomer was significantly reduced by an anti-TNF-α antibody, but the anti-TNF-β antibody had no effect. Our results suggest that the enzymatically depolymerized β-1,3-glucan oligomer induces TNF-α production from human monocytes." @default.
• W2065111539 created "2016-06-24" @default.
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• W2065111539 date "2003-01-01" @default.
• W2065111539 modified "2023-09-26" @default.
• W2065111539 title "Induction of TNF-α production from human peripheral blood monocytes with β-1,3-glucan oligomer prepared from laminarin with β-1,3-glucanase from Bacillus clausii NM-1" @default.
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• W2065111539 doi "https://doi.org/10.1016/s1389-1723(03)80128-7" @default.
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