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- W2065117009 abstract "A technique of continuous recording of the kinetics of biochemical reactions of nucleoside triphosphates by means of an ESR (electron spin resonance) method is proposed. The technique is based on the differential ability of NTP (nucleoside triphosphates) and the products of their conversion to coordinate Mn 2+ ions. Due to this fact the concentration of free (hydrated) Mn 2+ ions changes in the course of the reaction and, consequently, the intensity of their ESR signal also changes. The proposed technique makes it possible to determine changes of concentration ≳0.1% of the total free ion concentration. The technique was applied to observation of reactions catalyzed by RNA polymerase, alkaline phosphatase, and aminoacyl-tRNA synthetase." @default.
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- W2065117009 date "1977-02-01" @default.
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- W2065117009 title "Continuous electron spin resonance detection of biochemical reactions of nucleoside triphosphates" @default.
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- W2065117009 doi "https://doi.org/10.1016/0003-2697(77)90254-8" @default.
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