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• W2065130531 endingPage "422" @default.
• W2065130531 startingPage "400" @default.
• W2065130531 abstract "Considering the increasing necessity for improved preparation techniques in biological electron microscopy as a basis for the identification and localization of cellular substances within the compartments of the cell, this review is focussed on the method of freeze substitution as an important link between the cryofixation (ultrarapid freezing) and resin embedding of biological specimens. The theory and practice of freeze substitution is summarized with particular interest in the physical and thermodynamic as well as in the chemical basis of this technique. A survey of practical aspects of the technical process of freeze substitution concerning the equipment and various protocols successfully applied in biological systems is also given. The main advantage of freeze substitution versus conventional chemical fixation is seen in the maintenance of the hydration shell of molecules and macromolecular structures. This results in an improved fine structural preservation, superior retention of the antigenicity of proteins and decreased loss of unbound, diffusible cellular components. Examples of excellent visualization of the ultrastructure of macromolecular complexes (nucleic acids, extracellular material, membranes etc.), small organisms (bacteria, algae, cyanobacteria and fungi) and large biological samples such as plant and animal tissue as well as the plant-pathogen (fungus) interface and infection structures are presented. Recent data on the molecular characterization of freeze-substituted biological tissue are exemplified with special emphasis on the subcellular detection of soluble components (elements, lipids, proteins and drugs) and the inter-/intracellular localization of proteins including foreign proteins in transgenic plants. The molecular analysis of freeze-substituted specimens is achieved by the combination of low temperature preparation techniques in biological electron microscopy with various detection methods such as X-ray microanalysis, immunocytochemistry and high resolution autoradiography." @default.
• W2065130531 created "2016-06-24" @default.
• W2065130531 creator A5070181685 @default.
• W2065130531 date "1993-04-01" @default.
• W2065130531 modified "2023-10-02" @default.
• W2065130531 title "Impact of freeze substitution on biological electron microscopy" @default.
• W2065130531 cites W1155580745 @default.
• W2065130531 cites W122402334 @default.
• W2065130531 cites W127842574 @default.
• W2065130531 cites W1465301386 @default.
• W2065130531 cites W147519019 @default.
• W2065130531 cites W1482562733 @default.
• W2065130531 cites W1522556398 @default.
• W2065130531 cites W1545394260 @default.
• W2065130531 cites W1596855178 @default.
• W2065130531 cites W1695535299 @default.
• W2065130531 cites W1824036352 @default.
• W2065130531 cites W1836380042 @default.
• W2065130531 cites W1884738918 @default.
• W2065130531 cites W1914243789 @default.
• W2065130531 cites W1965907673 @default.
• W2065130531 cites W1966445035 @default.
• W2065130531 cites W1968085446 @default.
• W2065130531 cites W1968633848 @default.
• W2065130531 cites W1978603470 @default.
• W2065130531 cites W1979550452 @default.
• W2065130531 cites W1980074501 @default.
• W2065130531 cites W1984156890 @default.
• W2065130531 cites W1985054209 @default.
• W2065130531 cites W1988200446 @default.
• W2065130531 cites W1988503985 @default.
• W2065130531 cites W1989275107 @default.
• W2065130531 cites W1990688311 @default.
• W2065130531 cites W1991444534 @default.
• W2065130531 cites W1992333027 @default.
• W2065130531 cites W1993587057 @default.
• W2065130531 cites W1994010185 @default.
• W2065130531 cites W1994681837 @default.
• W2065130531 cites W1997220102 @default.
• W2065130531 cites W1997901956 @default.
• W2065130531 cites W2005569807 @default.
• W2065130531 cites W2009044908 @default.
• W2065130531 cites W2009761150 @default.
• W2065130531 cites W2009804711 @default.
• W2065130531 cites W2009881558 @default.
• W2065130531 cites W2009939144 @default.
• W2065130531 cites W2010218490 @default.
• W2065130531 cites W2015234027 @default.
• W2065130531 cites W2015505665 @default.
• W2065130531 cites W2015602070 @default.
• W2065130531 cites W2018134701 @default.
• W2065130531 cites W2020560356 @default.
• W2065130531 cites W2024783704 @default.
• W2065130531 cites W2026663918 @default.
• W2065130531 cites W2029517028 @default.
• W2065130531 cites W2029633472 @default.
• W2065130531 cites W2030236120 @default.
• W2065130531 cites W2030820525 @default.
• W2065130531 cites W2031789143 @default.
• W2065130531 cites W2032901228 @default.
• W2065130531 cites W2036415377 @default.
• W2065130531 cites W2042921800 @default.
• W2065130531 cites W2046649122 @default.
• W2065130531 cites W2049043548 @default.
• W2065130531 cites W2050209448 @default.
• W2065130531 cites W2053782594 @default.
• W2065130531 cites W2053982911 @default.
• W2065130531 cites W2055180005 @default.
• W2065130531 cites W2057240303 @default.
• W2065130531 cites W2060994706 @default.
• W2065130531 cites W2061017174 @default.
• W2065130531 cites W2061629333 @default.
• W2065130531 cites W2062256686 @default.
• W2065130531 cites W2062975178 @default.
• W2065130531 cites W2065865631 @default.
• W2065130531 cites W2069505560 @default.
• W2065130531 cites W2070069079 @default.
• W2065130531 cites W2070090382 @default.
• W2065130531 cites W2072502690 @default.
• W2065130531 cites W2076848477 @default.
• W2065130531 cites W2079437845 @default.
• W2065130531 cites W2079738104 @default.
• W2065130531 cites W2084098912 @default.
• W2065130531 cites W2085010310 @default.
• W2065130531 cites W2087213740 @default.
• W2065130531 cites W2088477395 @default.
• W2065130531 cites W2092120887 @default.
• W2065130531 cites W2103641169 @default.
• W2065130531 cites W2105773551 @default.
• W2065130531 cites W2106519555 @default.
• W2065130531 cites W2107230481 @default.
• W2065130531 cites W2109401564 @default.
• W2065130531 cites W2115220143 @default.
• W2065130531 cites W2134669086 @default.
• W2065130531 cites W2134746893 @default.
• W2065130531 cites W2139940907 @default.
• W2065130531 cites W2142784636 @default.
• W2065130531 cites W2151715319 @default.

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