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- W2065138001 abstract "A fiber-optic immunosensor was developed to measure the mycotoxin fumonisin B1 (FB1). Monoclonal antibodies produced against FB1 were covalently bound through a heterobifunctional silane to an etched 800 μm core optical fiber. An evanescent wave effect was utilized to excite fluorescein isothiocyanate labeled FB1 (FB1-FITC) molecules near the surface of the fiber. A direct competitive assay was used to measure FB1 concentrations with the following steps: saturation of antibody binding sites by FB1-FITC, competition of FB1 and FB1-FITC with displacement of the labeled toxin, and resaturation of binding sites by FB1-FITC. The signal generated in the assay was found to be inversely proportional to the FB1 concentration. This sensor exhibited a working range of 10−1000 ng of FB1/mL, an IC50 of 70 ng/mL, and a limit of detection of 10 ng/mL. These values compared favorably with those for currently available ELISA techniques. The sensor exhibited cross-reactivity with fumonisin B2 but did not react with hydrolyzed FB1, sphinganine, or tricarballylic acid. Sensor performance was also found to be unaffected in a sample matrix of methanol/water-extracted corn. Keywords: Immunosensor; fiber optic; fluorescence; fumonisin; biosensor" @default.
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- W2065138001 title "Fiber-Optic Immunosensor for the Detection of Fumonisin B<sub>1</sub>" @default.
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- W2065138001 doi "https://doi.org/10.1021/jf950661t" @default.
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