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• W2065180810 abstract "Somatic mutation calling from next-generation sequencing data remains a challenge due to the difficulties of distinguishing true somatic events from artifacts arising from PCR, sequencing errors or mis-mapping. Tumor cellularity or purity, sub-clonality and copy number changes also confound the identification of true somatic events against a background of germline variants. We have developed a heuristic strategy and software (http://www.qcmg.org/bioinformatics/qsnp/) for somatic mutation calling in samples with low tumor content and we show the superior sensitivity and precision of our approach using a previously sequenced cell line, a series of tumor/normal admixtures, and 3,253 putative somatic SNVs verified on an orthogonal platform." @default.
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• W2065180810 date "2013-11-08" @default.
• W2065180810 modified "2023-10-16" @default.
• W2065180810 title "Somatic Point Mutation Calling in Low Cellularity Tumors" @default.
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• W2065180810 doi "https://doi.org/10.1371/journal.pone.0074380" @default.
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