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- W2065193886 abstract "1. [14C]Chloramphenicol is taken up by intact Bacillus megaterium when incubated in a suitable growth medium. In a cell-free system containing either K+ or NH+4, the antibiotic is bound specifically to the 50-S subunit of the ribosomes. 2. The uptake of [14C]chloramphenicol by B. megaterium and its binding to bacterial ribosomes is prevented by d-thiocymetin, d-win 5094, streptogramin, streptogramin A, lincomycin, celesticetin and all seven macrolide antibiotics tested. Streptogramin B, staphylomycin S and viridogrisein reduce the uptake of [14C]-chloramphenicol by intact bacteria but have no effect on the binding of [14C]chloramphenicol to ribosomes in cell-free systems. 3. The effects of some of the compounds on the ribosome are reversible whereas other antibiotics appear to affect the ribosome irreversibly." @default.
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- W2065193886 date "1966-02-01" @default.
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- W2065193886 title "Binding of chloramphenicol to ribosomes The effect of a number of antibiotics" @default.
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- W2065193886 doi "https://doi.org/10.1016/0005-2787(66)90309-1" @default.
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