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- W2065200488 endingPage "e7050" @default.
- W2065200488 startingPage "e7050" @default.
- W2065200488 abstract "Nuclear lamin filaments and associated proteins form a nucleoskeletal (lamina) network required for transcription, replication, chromatin organization and epigenetic regulation in metazoans. Lamina defects cause human disease (laminopathies) and are linked to aging. Barrier-to-autointegration factor (BAF) is a mobile and essential component of the nuclear lamina that binds directly to histones, lamins and LEM-domain proteins, including the inner nuclear membrane protein emerin, and has roles in chromatin structure, mitosis and gene regulation. To understand BAF's mechanisms of action, BAF associated proteins were affinity-purified from HeLa cell nuclear lysates using BAF-conjugated beads, and identified by tandem mass spectrometry or independently identified and quantified using the iTRAQ method. We recovered A- and B-type lamins and core histones, all known to bind BAF directly, plus four human transcription factors (Requiem, NonO, p15, LEDGF), disease-linked proteins (e.g., Huntingtin, Treacle) and several proteins and enzymes that regulate chromatin. Association with endogenous BAF was independently validated by co-immunoprecipitation from HeLa cells for seven candidates including Requiem, poly(ADP-ribose) polymerase 1 (PARP1), retinoblastoma binding protein 4 (RBBP4), damage-specific DNA binding protein 1 (DDB1) and DDB2. Interestingly, endogenous BAF and emerin each associated with DDB2 and CUL4A in a UV- and time-dependent manner, suggesting BAF and emerin have dynamic roles in genome integrity and might help couple DNA damage responses to the nuclear lamina network. We conclude this proteome is a rich source of candidate partners for BAF and potentially also A- and B-type lamins, which may reveal how chromatin regulation and genome integrity are linked to nuclear structure." @default.
- W2065200488 created "2016-06-24" @default.
- W2065200488 creator A5004066155 @default.
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- W2065200488 creator A5054004495 @default.
- W2065200488 creator A5058384217 @default.
- W2065200488 date "2009-09-16" @default.
- W2065200488 modified "2023-10-18" @default.
- W2065200488 title "Barrier-to-Autointegration Factor Proteome Reveals Chromatin-Regulatory Partners" @default.
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