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• W2065224272 abstract "We describe the cloning, sequencing and overexpression of the (3–17)β hydroxysteroid dehydrogenase gene of Pseudomonas testosteroni. A genomic library of Ps. testosteroni total DNA constructed from SauIIIA digests ligated to a λgt11 vector was probed with polyclonal antibody raised against purified enzyme. Subclones derived from a recombinant phage containing a 2661-base-pair insert were sequenced and found to contain an open reading frame of 765 base pairs that corresponds to a protein of 254 amino acid residues. A 1492-base-pair fragment was inserted into pBR322 plasmid vector and used to construct a strain of E. coli HB101 that overexpressed the steroid dehydrogenase gene." @default.
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• W2065224272 date "1993-02-01" @default.
• W2065224272 modified "2023-09-25" @default.
• W2065224272 title "Cloning, DNA sequencing and expression of (3–17)β hydroxysteroid dehydrogenase from Pseudomonas testosteroni" @default.
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• W2065224272 doi "https://doi.org/10.1016/0960-0760(93)90020-w" @default.
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