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- W2065258210 abstract "Abstract A synthetic medium was developed in which the presence of phenylalanine ammonialyase (PAL) in the yeast Rhodotorula glutinis was dependent on the addition of l -phenylalanine. The appearance of PAL activity occurred during mid- to late log phase regardless of the time of l -phenylalanine introduction into the medium. Maximum levels of PAL activity were followed by a rapid decline in both total and specific activity. These changes were accompanied by comparable fluctuations in PAL antigen levels as measured by rocket immunoelectrophoresis. Proteins of yeast grown in the presences of l -phenylalanine were radiolabeled in vivo with l -[ 3 H]leucine. The labeled protein was immunoprecipitated with anti-PAL serum and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A labeled protein comigrated with subunits of authentic PAL. These data support the hypothesis that de novo synthesis of PAL in R. glutinis is l -phenylalanine dependent." @default.
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- W2065258210 date "1982-07-01" @default.
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- W2065258210 title "Phenylalanine-dependent de novo synthesis of phenylalanine ammonia-lyase from Rhodotorula glutinis" @default.
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- W2065258210 doi "https://doi.org/10.1016/0003-9861(82)90226-0" @default.
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