Matches in SemOpenAlex for { <https://semopenalex.org/work/W2065291274> ?p ?o ?g. }
- W2065291274 endingPage "590" @default.
- W2065291274 startingPage "585" @default.
- W2065291274 abstract "By integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device, we have developed a microanalytical platform for performing electrophoresis-based immunoassays. The microfluidic immunoassays are performed by gel electrophoretic separation and quantitation of bound and unbound antibody or antigen. To retain biological activity of proteins and maintain intact immune complexes, nondenaturing polyacrylamide gel electrophoresis conditions were investigated. Both direct (noncompetitive) and competitive immunoassay formats are demonstrated in microchips. A direct immunoassay was developed for detection of tetanus antibodies in buffer as well as diluted serum samples. After an off-chip incubation step, the immunoassay was completed in less than 3 min and the sigmoidal dose−response curve spanned an antibody concentration range from 0.17 to 260 nM. The minimum detectable antibody concentration was 0.68 nM. A competitive immunoassay was also developed for tetanus toxin C-fragment by allowing unlabeled and fluorescently labeled tetanus toxin C-fragment compete to bind to a limited fixed concentration of tetanus antibody. The immunoassay technique described in this work shows promise as a component of an integrated microfluidic device amenable to automation and relevant to development of clinical diagnostic devices." @default.
- W2065291274 created "2016-06-24" @default.
- W2065291274 creator A5041375274 @default.
- W2065291274 creator A5043724778 @default.
- W2065291274 creator A5046265360 @default.
- W2065291274 creator A5049492000 @default.
- W2065291274 date "2004-12-03" @default.
- W2065291274 modified "2023-09-26" @default.
- W2065291274 title "On-Chip Native Gel Electrophoresis-Based Immunoassays for Tetanus Antibody and Toxin" @default.
- W2065291274 cites W1829459140 @default.
- W2065291274 cites W1964139393 @default.
- W2065291274 cites W1972466773 @default.
- W2065291274 cites W1975507264 @default.
- W2065291274 cites W1984099739 @default.
- W2065291274 cites W1999947472 @default.
- W2065291274 cites W2003614295 @default.
- W2065291274 cites W2009308705 @default.
- W2065291274 cites W2011369587 @default.
- W2065291274 cites W2039025097 @default.
- W2065291274 cites W2047725750 @default.
- W2065291274 cites W2052457044 @default.
- W2065291274 cites W2054631692 @default.
- W2065291274 cites W2065642993 @default.
- W2065291274 cites W2069829737 @default.
- W2065291274 cites W2074041522 @default.
- W2065291274 cites W2084786239 @default.
- W2065291274 cites W2085101416 @default.
- W2065291274 cites W2106064918 @default.
- W2065291274 cites W2132119662 @default.
- W2065291274 doi "https://doi.org/10.1021/ac0489768" @default.
- W2065291274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15649057" @default.
- W2065291274 hasPublicationYear "2004" @default.
- W2065291274 type Work @default.
- W2065291274 sameAs 2065291274 @default.
- W2065291274 citedByCount "76" @default.
- W2065291274 countsByYear W20652912742012 @default.
- W2065291274 countsByYear W20652912742013 @default.
- W2065291274 countsByYear W20652912742014 @default.
- W2065291274 countsByYear W20652912742015 @default.
- W2065291274 countsByYear W20652912742016 @default.
- W2065291274 countsByYear W20652912742018 @default.
- W2065291274 countsByYear W20652912742019 @default.
- W2065291274 countsByYear W20652912742020 @default.
- W2065291274 countsByYear W20652912742021 @default.
- W2065291274 countsByYear W20652912742022 @default.
- W2065291274 crossrefType "journal-article" @default.
- W2065291274 hasAuthorship W2065291274A5041375274 @default.
- W2065291274 hasAuthorship W2065291274A5043724778 @default.
- W2065291274 hasAuthorship W2065291274A5046265360 @default.
- W2065291274 hasAuthorship W2065291274A5049492000 @default.
- W2065291274 hasConcept C101660505 @default.
- W2065291274 hasConcept C116084860 @default.
- W2065291274 hasConcept C159654299 @default.
- W2065291274 hasConcept C171250308 @default.
- W2065291274 hasConcept C181199279 @default.
- W2065291274 hasConcept C185592680 @default.
- W2065291274 hasConcept C192562407 @default.
- W2065291274 hasConcept C203014093 @default.
- W2065291274 hasConcept C2777367657 @default.
- W2065291274 hasConcept C2780420688 @default.
- W2065291274 hasConcept C40250595 @default.
- W2065291274 hasConcept C43617362 @default.
- W2065291274 hasConcept C55493867 @default.
- W2065291274 hasConcept C8673954 @default.
- W2065291274 hasConcept C86803240 @default.
- W2065291274 hasConceptScore W2065291274C101660505 @default.
- W2065291274 hasConceptScore W2065291274C116084860 @default.
- W2065291274 hasConceptScore W2065291274C159654299 @default.
- W2065291274 hasConceptScore W2065291274C171250308 @default.
- W2065291274 hasConceptScore W2065291274C181199279 @default.
- W2065291274 hasConceptScore W2065291274C185592680 @default.
- W2065291274 hasConceptScore W2065291274C192562407 @default.
- W2065291274 hasConceptScore W2065291274C203014093 @default.
- W2065291274 hasConceptScore W2065291274C2777367657 @default.
- W2065291274 hasConceptScore W2065291274C2780420688 @default.
- W2065291274 hasConceptScore W2065291274C40250595 @default.
- W2065291274 hasConceptScore W2065291274C43617362 @default.
- W2065291274 hasConceptScore W2065291274C55493867 @default.
- W2065291274 hasConceptScore W2065291274C8673954 @default.
- W2065291274 hasConceptScore W2065291274C86803240 @default.
- W2065291274 hasIssue "2" @default.
- W2065291274 hasLocation W20652912741 @default.
- W2065291274 hasLocation W20652912742 @default.
- W2065291274 hasOpenAccess W2065291274 @default.
- W2065291274 hasPrimaryLocation W20652912741 @default.
- W2065291274 hasRelatedWork W1966102502 @default.
- W2065291274 hasRelatedWork W1990549102 @default.
- W2065291274 hasRelatedWork W2037307292 @default.
- W2065291274 hasRelatedWork W2038266014 @default.
- W2065291274 hasRelatedWork W2049881746 @default.
- W2065291274 hasRelatedWork W2050407748 @default.
- W2065291274 hasRelatedWork W2988700287 @default.
- W2065291274 hasRelatedWork W4235238160 @default.
- W2065291274 hasRelatedWork W2014643914 @default.
- W2065291274 hasRelatedWork W2183959742 @default.
- W2065291274 hasVolume "77" @default.
- W2065291274 isParatext "false" @default.
- W2065291274 isRetracted "false" @default.