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- W2065299398 abstract "An unknown phosphate-containing compound has been isolated from the sartorius muscle of the toad. The compound was detected on paper chromatograms run to isolate the ademine nucleotides and creatine phosphate. Using solvent systems usually employed to separate ATP and ADP it was found to have an RF value identical to that of ADP, and consequently was routinely estimated with it. This finding accounts for the high values of ADP reported by us, taking account of the levels of creatine phosphate known to be present in muscle, and the activity of the creatine phosphoryltransferase system. The compound was found not to be a nucleotide, or a purine or pyrimidine derivative. Microanalysis showed it to contain C, N, H, orthophosphate and a large but constant proportion of non-combustible ash. Al was the principal metal contained in the ash. Evidence from infrared and ultraviolet spectra suggests that the compound contains an unsaturated heterocyclic ring. The Al may be present as a metal chelate with the phosphate moiety. The phosphate group of the compound failed to exchange with 32P-labelled orthophosphate under conditions when there was a substantial exchange of the other acid-soluble phosphate compounds of muscle. No information is available as to the possible biological function of the compound." @default.
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- W2065299398 date "1964-07-01" @default.
- W2065299398 modified "2023-09-24" @default.
- W2065299398 title "An unknown phosphate compound in muscle" @default.
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- W2065299398 doi "https://doi.org/10.1016/0304-4165(64)90117-5" @default.
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