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- W2065306095 abstract "Recently, we have reported that theophylline concentrations in plasma as determined by substratelabeled fluorescent immunoassay (SLFIA) well correlated (r= 0.96) with the concentrations by high-pressure liquid chromatography, and that SLFIA procedure had considerable advantages over hitherto available method. Methods for measurin.g theophylline in saliva previously reported were spectrophotometry, gas-liquid and high-pressure liquid chromatographies. Here, we used the SLFIA for the determination of theophylline concentrations in saliva. It was possible to determine salivary theophylline in low concentrations by using salivary altered first dilution rates with SLFIA method. The concentrations of theophylline in the plasma and saliva in 7 normal adults and 3 asthmatic patients who were administered aminophylline were determined by SLFIA method. In normal adults, the time-course of plasma and salivary theophylline levels showed a parallel relationship, and theophylline concentrations in the saliva as determined 2 and 4 hours after administration were about 44% lower than those in the plasma. In asthmatic patients, theophylline concentrations in saliva 2, 4, 6 and 8 hours after administration were also 32% lower than those in the plasma. These results suggested that monitoring patients by means of salivary theophylline level is effective way to predict plasma levels and to help patients avoid pain of bloodletting." @default.
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- W2065306095 date "1982-01-01" @default.
- W2065306095 modified "2023-09-24" @default.
- W2065306095 title "Determination of Salivary Theophylline Concentration by SLFIA Method and Pharmacokinetics in Saliva" @default.
- W2065306095 doi "https://doi.org/10.5649/jjphcs1975.8.144" @default.
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